Revision as of 01:20, 30 October 2008

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Things we did today

Grace

EtBr stained 2% agarose gel ran at 95V for 1 hour. Three microliters of PCR product were loaded into each lane.

**Colony Count of J33207+pRL1383a Transformants**
Plate	Cells	Colonies	Blue?
LB+Amp₁₀₀X-gal (+) control	J33207 in pSB1A2 in DH5α	too many to count, medium	yes
LB+sp₁₀₀X-gal (+) control	BBpRL1383a-1 (10/25 inoculation plasmid prep) in DH5α	too many to count, small	no
LB+X-gal (+) control	untransformed DH5α	lawn/smear	no
LB+sp_2.5+X-gal (-) control	untransformed DH5α	0	no
LB+sp₁₀₀ (-) control	untransformed DH5α	0	no
LB+sp_2.5	J33207+pRL1383a ligation in DH5α	lawn/smear	no
LB+sp₅	J33207+pRL1383a ligation in DH5α	lawn/smear	no
LB+sp₁₀	J33207+pRL1383a ligation in DH5α	lawn/smear	no
LB+sp₂₀	J33207+pRL1383a ligation in DH5α	lawn/smear	no
LB+sp₂₅	J33207+pRL1383a ligation in DH5α	lawn/smear	no
LB+sp₄₀	J33207+pRL1383a ligation in DH5α	too many to count, small	no
LB+sp₅₀	J33207+pRL1383a ligation in DH5α	too many to count, small to medium	no
LB+sp₇₅	J33207+pRL1383a ligation in DH5α	too many to count, small to medium	no
LB+sp₁₀₀	J33207+pRL1383a ligation in DH5α	too many to count, small to medium	no

Grace

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

@@ Line 13: / Line 13: @@
 !Blue?
 |-
-|align=center|LB+Amp<sub>100</sub>X-gal (+) control
+|align=center|LB+Amp<sub>100</sub>X-gal <br>(+) control
 |align=center|J33207 in pSB1A2 in DH5&alpha;
 |align=center|too many to count, medium
 |align=center|yes
 |-
-|align=center|LB+sp<sub>100</sub>X-gal (+) control
+|align=center|LB+sp<sub>100</sub>X-gal <br>(+) control
-|align=center|BBpRL1383a-1 (10/25 inoculation plasmid prep) in DH5&alpha;
+|align=center|BBpRL1383a-1 <br>(10/25 inoculation plasmid prep) in DH5&alpha;
 |align=center|too many to count, small
 |align=center|no
 |-
-|align=center|LB+X-gal (+) control
+|align=center|LB+X-gal <br>(+) control
 |align=center|untransformed DH5&alpha;
 |align=center|lawn/smear
 |align=center|no
 |-
-|align=center|LB+sp<sub>2.5</sub>+X-gal (-) control
+|align=center|LB+sp<sub>2.5</sub>+X-gal <br>(-) control
 |align=center|untransformed DH5&alpha;
 |align=center|0
 |align=center|no
 |-
-|align=center|LB+sp<sub>100</sub> (-) control
+|align=center|LB+sp<sub>100</sub> <br>(-) control
 |align=center|untransformed DH5&alpha;
 |align=center|0