Team:Imperial College/Biobricks

From 2008.igem.org

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{{Imperial/Box2|Biobrick Characterisation in ''B.subtilis''|
{{Imperial/Box2|Biobrick Characterisation in ''B.subtilis''|
This year Imperial College has added 45 new ''B.subtilis'' specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''. The testing constructs we developed for this are shown below:
This year Imperial College has added 45 new ''B.subtilis'' specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site '''pveg-spoVG'''. The testing constructs we developed for this are shown below:
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[[Image:Biobricks Imperial.PNG|center|500px]]
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{{Imperial/Box1|Summary of Data|
{{Imperial/Box1|Summary of Data|
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[[Image:Biobricks Imperial.PNG|center|500px]]
 
[[Image:Fluorescence B.subtilis1.PNG]]
[[Image:Fluorescence B.subtilis1.PNG]]
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Revision as of 20:38, 29 October 2008


Biobrick Characterisation in B.subtilis

This year Imperial College has added 45 new B.subtilis specific parts to the registry. In addition we sort to characterise some of these parts, including the constitutive promoter and ribosome binding site pveg-spoVG. The testing constructs we developed for this are shown below:

Biobricks Imperial.PNG


Summary of Data

The promoter and RBS combinations were characterised by measuring the steadty-state levels expression of fluorescent proteins in B.subtilis. Cultures of B.subtilis transformed with the test constructs and non-transformed B.subtilis (control) were grown to the mid-log phase and measured using a plate reader.



Fluorescence B.subtilis1.PNG
Fluorescence B.subtilis.PNG


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