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Team:Imperial College/CAT
From 2008.igem.org
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| + | ===Chloramphenicol Acetyltransferase Characterisation=== | ||
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Characterisation of the chloramphenicol concentrations at which chlorampehnicol acetyltransferase ([http://partsregistry.org/wiki/index.php?title{{equals}}Part:BBa_J31005 Part: BBa_J31005]) (CAT) will protect cells, allowing them to grow and thrive is essentially to allow the correct selection when using the CAT gene. | Characterisation of the chloramphenicol concentrations at which chlorampehnicol acetyltransferase ([http://partsregistry.org/wiki/index.php?title{{equals}}Part:BBa_J31005 Part: BBa_J31005]) (CAT) will protect cells, allowing them to grow and thrive is essentially to allow the correct selection when using the CAT gene. | ||
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To this end, characterisation of chloramphenicol acetyl transferase was carried out according to this [https://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Resistance protocol] | To this end, characterisation of chloramphenicol acetyl transferase was carried out according to this [https://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Resistance protocol] | ||
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| + | {{Imperial/Box2|| | ||
| + | ==Results== | ||
| + | [Image:CAT_Results.PNG|width=80%] | ||
| + | [Image:CAT_Working_Concentration.PNG|width=80%] | ||
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| + | The construct containing the CAT gene was integrate into the ''B. subtilis'' genome for characterisation. As such, the CAT gene is single copy only. CAT will be able to protect a cell from higher levels of chloramphenicol if it is present in a construct on a plasmid (particularly a high copy number plasmid) | ||
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| + | <br><hr>{{Imperial/EndPage|Major_Results|Major_Results}} | ||
Revision as of 22:56, 29 October 2008
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Chloramphenicol Acetyltransferase Characterisation
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