Team:Imperial College/Genetic Circuit
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The pre-steady-state dynamic behaviour of the GFP concentration will differ with different initial concentrations of IPTG (but the steady-state behaviour will not). Hence, accuarate data collection during the pre-steady-state phase is crucial for paramater estimation. | The pre-steady-state dynamic behaviour of the GFP concentration will differ with different initial concentrations of IPTG (but the steady-state behaviour will not). Hence, accuarate data collection during the pre-steady-state phase is crucial for paramater estimation. | ||
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+ | A more sophisticated model allows for interaction between IPTG and the promoter-LacI complex <CITATION NEEDED>. Under this model, the dynamic behaviour (whether or not [GFP] attains a maximum higher than its steady-state value) depends on the relative strengths of the kinetic constants describing the interactions underlying the model. Either way, the steady-state [GFP] will vary as a Hill-function dependent on the initial concentration of IPTG; this characteristic can be used to discriminate between the two models. | ||
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+ | [[Media:InduciblePromoter_ODE.m|ODEs]]<br> | ||
+ | [[Media:InduciblePromoter.m|Simulation File]] | ||
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{{Imperial/EndPage|Growth_Curve|Motility}} | {{Imperial/EndPage|Growth_Curve|Motility}} |
Revision as of 16:03, 21 October 2008
Modelling the Genetic Circuit
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