Team:Imperial College/Protocols

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Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!

{{Imperial/Box1|Protocols|

1. Transformation Protocols for integration vectors in Bacillus subtilis

2. Transformation Protocol for linear DNA in Bacillus subtilis

[[/Transformation_3 |Protocol

3. Transformation Protocol E.coli

Preparation of XL1-blue electrocompetent cells

4. Motility Analysis

Motility Assays

5. PCR

6. Calibration Curves

7. Promoter-RBS Testing Protocols

8. Biomaterial Protocols

Biomaterial Assay

|}}

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 {{Imperial/Box1|Protocols|
 ====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======
-*[[/Transformation_Protocol | Protocol 1]]
+*[[/Transformation |Protocol 1]]
-*[[/Transformation_Protocol_2 | Protocol 2]]
+*[[/Transformation_2 |Protocol 2]]
 ====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======
-*Protocol
+*[[/Transformation_3 |Protocol
 ====== 3. Transformation Protocol ''E.coli'' ======
-*[[/XL1_Blue | Preparation of XL1-blue electrocompetent cells]]
+*[[/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]
 ====== 4. Motility Analysis ======
-*[[/Motility_Assay | Motility Assays]]
+*[[/Motility_Assay |Motility Assays]]
 ====== 5. PCR ======
-*[[/PCR| PCR]]
+*[[/PCR |PCR]]
-*[[/SCPCR| Single Colony PCR]]
+*[[/SCPCR |Single Colony PCR]]
 ====== 6. Calibration Curves ======
-*[[/Calibration_Curves| Calibration Curves ]]
+*[[/Calibration_Curves |Calibration Curves ]]
-*[[/Growth| O.D.<sub>600</sub> vs cell number]]
+*[[/Growth |O.D.<sub>600</sub> vs cell number]]
-*[[/Fluorescence| Fluorescence vs GFPmut3b]]
+*[[/Fluorescence |Fluorescence vs GFPmut3b]]
 ====== 7. Promoter-RBS Testing Protocols ======
-*[[/Constitutive | Constitutive Promoters and RBS]]
+*[[/Constitutive |Constitutive Promoters and RBS]]
-*[[/Inducible | pHyperspank Promoter and RBS]]
+*[[/Inducible |pHyperspank Promoter and RBS]]
-*[[/Light_Inducible | Light Inducible Promoters and RBS]]
+*[[/Light_Inducible |Light Inducible Promoters and RBS]]
 ====== 8. Biomaterial Protocols ======
 *[[/Biomaterial_Assay |Biomaterial Assay]]