Team:Imperial College/Protocols

From 2008.igem.org

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====== 6. Calibration Curves ======
====== 6. Calibration Curves ======
*[[Team:Imperial  College/Calibration_Curves |Calibration Curves ]]
*[[Team:Imperial  College/Calibration_Curves |Calibration Curves ]]
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*[[Team:Imperial  College/Growth |O.D.<sub>600</sub> vs cell number]]
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*[[Team:Imperial  College/Growth |O.D <sub>600</sub> vs cell number]]
*[[Team:Imperial  College/Fluorescence |Fluorescence vs GFPmut3b]]
*[[Team:Imperial  College/Fluorescence |Fluorescence vs GFPmut3b]]
====== 7. Promoter-RBS Testing Protocols ======
====== 7. Promoter-RBS Testing Protocols ======

Revision as of 18:18, 26 October 2008

Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!


Protocols
1. Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for linear DNA in Bacillus subtilis
3. Transformation Protocol for E.coli
4. Motility Analysis
5. PCR
6. Calibration Curves
7. Promoter-RBS Testing Protocols
8. Biomaterial Protocols