http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&feed=atom&action=history
Team:Imperial College/Protocols - Revision history
2024-03-29T11:30:55Z
Revision history for this page on the wiki
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http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=95664&oldid=prev
Saint at 21:14, 29 October 2008
2008-10-29T21:14:26Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Transformation |Protocol 1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Transformation |Protocol 1]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Transformation_2 |Protocol 2]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Transformation_2 |Protocol 2]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== 2<del class="diffchange diffchange-inline">. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== 2. Transformation Protocol for ''E.coli'' ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[[Team:Imperial College/Transformation_3 |Protocol]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">====== 3</del>. Transformation Protocol for ''E.coli'' ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">4</del>. PCR ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">3</ins>. PCR ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">5</del>. Calibration Curves ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">4</ins>. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">6</del>. Promoter-RBS Testing Protocols ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">5</ins>. Promoter-RBS Testing Protocols ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">7. Biomaterial Protocols ======</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">6</ins>. Antibiotic Resistance Testing ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[[Team:Imperial College/Biomaterial_Assay |Biomaterial Detection]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">====== 8</del>. Antibiotic Resistance Testing ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td></tr>
</table>
Saint
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=92146&oldid=prev
Qiqin at 17:19, 29 October 2008
2008-10-29T17:19:32Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 17:19, 29 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Biomaterial Protocols ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Biomaterial Protocols ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial <del class="diffchange diffchange-inline">Assay</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial <ins class="diffchange diffchange-inline">Detection</ins>]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 8. Antibiotic Resistance Testing ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 8. Antibiotic Resistance Testing ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]]</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=84298&oldid=prev
Chris at 01:34, 29 October 2008
2008-10-29T01:34:19Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Biomaterial Protocols ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Biomaterial Protocols ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial Assay]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial Assay]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">====== 8. Antibiotic Resistance Testing ======</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*[[Team:Imperial College/Resistance |Chloramphenicol Resistance Assay]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td></tr>
</table>
Chris
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=84289&oldid=prev
Chris at 01:31, 29 October 2008
2008-10-29T01:31:57Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. PCR ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. PCR ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">*[[Team:Imperial College/SCPCR |Single Colony PCR]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. Calibration Curves ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td></tr>
</table>
Chris
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=81642&oldid=prev
Qiqin at 20:12, 28 October 2008
2008-10-28T20:12:06Z
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/StartPage2}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/StartPage2}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Experiments & Protocols ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Experiments & Protocols ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/Box2||</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/Box2||</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=80138&oldid=prev
Qiqin at 17:40, 28 October 2008
2008-10-28T17:40:15Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== 4<del class="diffchange diffchange-inline">. Motility Analysis ======</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== 4. PCR ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*[[Team:Imperial College/Motility_Assay |Motility Assays]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">====== 5</del>. PCR ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/PCR |PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/SCPCR |Single Colony PCR]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/SCPCR |Single Colony PCR]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">6</del>. Calibration Curves ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">5</ins>. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">7</del>. Promoter-RBS Testing Protocols ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">6</ins>. Promoter-RBS Testing Protocols ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Constitutive |Constitutive Promoters and RBS]] </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Inducible |pHyperspank Promoter and RBS]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Light_Inducible |Light Inducible Promoters and RBS]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>====== <del class="diffchange diffchange-inline">8</del>. Biomaterial Protocols ======</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>====== <ins class="diffchange diffchange-inline">7</ins>. Biomaterial Protocols ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial Assay]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Biomaterial_Assay |Biomaterial Assay]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=68924&oldid=prev
Qiqin at 18:18, 26 October 2008
2008-10-26T18:18:46Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:18, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Calibration_Curves |Calibration Curves ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D<del class="diffchange diffchange-inline">.</del><sub>600</sub> vs cell number]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Growth |O.D <sub>600</sub> vs cell number]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Team:Imperial College/Fluorescence |Fluorescence vs GFPmut3b]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Promoter-RBS Testing Protocols ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Promoter-RBS Testing Protocols ======</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=67765&oldid=prev
Qiqin at 14:41, 26 October 2008
2008-10-26T14:41:55Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:41, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/Box1|Protocols|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Imperial/Box1|Protocols|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/Transformation |Protocol 1]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/Transformation |Protocol 1]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/Transformation_2 |Protocol 2]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/Transformation_2 |Protocol 2]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Transformation_3 |Protocol]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Transformation_3 |Protocol]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. Motility Analysis ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. Motility Analysis ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Motility_Assay |Motility Assays]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Motility_Assay |Motility Assays]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. PCR ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. PCR ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/PCR |PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/PCR |PCR]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/SCPCR |Single Colony PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/SCPCR |Single Colony PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[Imperial College/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:</ins>Imperial <ins class="diffchange diffchange-inline"> </ins>College/Calibration_Curves |Calibration Curves ]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Growth |O.D.<sub>600</sub> vs cell number]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Growth |O.D.<sub>600</sub> vs cell number]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Fluorescence |Fluorescence vs GFPmut3b]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Fluorescence |Fluorescence vs GFPmut3b]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Promoter-RBS Testing Protocols ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 7. Promoter-RBS Testing Protocols ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Constitutive |Constitutive Promoters and RBS]] </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Constitutive |Constitutive Promoters and RBS]] </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Inducible |pHyperspank Promoter and RBS]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Inducible |pHyperspank Promoter and RBS]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Light_Inducible |Light Inducible Promoters and RBS]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Light_Inducible |Light Inducible Promoters and RBS]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 8. Biomaterial Protocols ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 8. Biomaterial Protocols ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Biomaterial_Assay |Biomaterial Assay]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Team:Imperial College</ins>/Biomaterial_Assay |Biomaterial Assay]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><hr>{{Imperial/EndPage|Cloning_Strategy|BioBricks}}</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=67753&oldid=prev
Qiqin at 14:38, 26 October 2008
2008-10-26T14:38:47Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:38, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Transformation_3 |Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Transformation_3 |Protocol]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 3. Transformation Protocol for ''E.coli'' ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Imperial College</ins>/XL1_Blue |Preparation of XL1-blue electrocompetent cells]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. Motility Analysis ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 4. Motility Analysis ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Motility_Assay |Motility Assays]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Motility_Assay |Motility Assays]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. PCR ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 5. PCR ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/PCR |PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Imperial College</ins>/PCR |PCR]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/SCPCR |Single Colony PCR]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Imperial College</ins>/SCPCR |Single Colony PCR]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 6. Calibration Curves ======</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Calibration_Curves |Calibration Curves ]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Imperial College</ins>/Calibration_Curves |Calibration Curves ]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Growth |O.D.<sub>600</sub> vs cell number]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Growth |O.D.<sub>600</sub> vs cell number]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Fluorescence |Fluorescence vs GFPmut3b]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Fluorescence |Fluorescence vs GFPmut3b]]</div></td></tr>
</table>
Qiqin
http://2008.igem.org/wiki/index.php?title=Team:Imperial_College/Protocols&diff=67742&oldid=prev
Qiqin at 14:36, 26 October 2008
2008-10-26T14:36:51Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:36, 26 October 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 1. Transformation Protocols for integration vectors in ''Bacillus subtilis'' ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Imperial College/Transformation |Protocol 1]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[Imperial College/Transformation |Protocol 1]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*[[/Transformation_2 |Protocol 2]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*[[<ins class="diffchange diffchange-inline">Imperial College</ins>/Transformation_2 |Protocol 2]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====== 2. Transformation Protocol for linear DNA in ''Bacillus subtilis'' ======</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Transformation_3 |Protocol]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*[[/Transformation_3 |Protocol]]</div></td></tr>
</table>
Qiqin