Team:Johns Hopkins/Notebook/GROUP 1: Fluorescent Proteins

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GROUP 1: Fluorescent Proteins

 Date: July 1 2008
 status report by: Ingrid (work done by James)
 Part no.: BBa_K110017
 Part Description: yESapphire 
 Part Location (in build a genome lab): In James and Jasper's PCR product Box,
 Stainless Steel 4 degree
 PCR successful?; Yes
 Cloning of PCR product successful: Y/N
 Sequencing of cloned PCR product successful: Not done
 Joining of validated part to adjacent part(s) status: Not done
 Current status of this part: This part is being Sequenced
 Date: July 1 2008
 status report by: Ingrid (work done by James)
 Part no.: BBa_K110010
 Part Description: yESapphire 
 Part Location (in build a genome lab): In James and Jasper's PCR product Box, 
 Stainless Steel 4 degree
 PCR successful?; Yes
 Cloning of PCR product successful: Y/N
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Current status of this part: This part is being sequenced


 Date: July 17 2008
 status report by: Ingrid 
 Part no.: BBa_K110018 
 Part Description: mCherry: 
 Part Location (in build a genome lab): In silver fridge by door
 PCR successful?; Yes 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP]
 Cloning of PCR product successful: Inconclusive 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest]
 Sequencing of cloned PCR product successful: Not done
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved: Had some extra unknown products, with unknown bands in the gel
 Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
 Date: July 17 2008
 status report by: Ingrid 
 Part no.: BBa_K110019
 Part Description: mCherry: 
 Part Location (in build a genome lab): In silver fridge by door
 PCR successful?; Yes 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP]
 Cloning of PCR product successful: Inconclusive 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest]
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved: Need to figure out if gel after digest shows correct products
 Date: July 17 2008
 status report by: Ingrid 
 Part no.: BBa_K110020 
 Part Description: Venus Enhanced YFP
 Part Location (in build a genome lab): In silver fridge by door
 PCR successful?; Yes 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP]
 Cloning of PCR product successful: inconclusive
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest]
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved: Had some extra unknown products, with unknown bands in the gel
 Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.
 Date: July 17 2008
 status report by: Ingrid 
 Part no.: BBa_K110021 
 Part Description: Venus Enhanced YFP
 Part Location (in build a genome lab): In silver fridge by door
 PCR successful?; Yes 
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.PCR%20Products%20of%20mCherry%20and%20vYFP.Ingrid%20&%20Tejas.html PCR Products of mCherry and vYFP]
 Cloning of PCR product successful: Inconclusive
 [http://www.jhu.edu/iGEM/Group1:FluorescentProteins/2008-7-22.Venus%20YFP%20and%20mCherry%20Miniprep%20check%20via%20digest.Ingrid.html Venus YFP and mCherry Miniprep check via digest]
 Sequencing of cloned PCR product successful: No
 Joining of validated part to adjacent part(s) status: Not done
 Problems to be solved: Had some extra unknown products, with unknown bands in the gel
 Current status of this part: Done with touchdown PCR and gel. Next step is to clone product.