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Team:KULeuven/13 August 2008
From 2008.igem.org
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Lab Work
Wet Lab
- We tested the ligations (using PCR and gel electrophoresis) of the following parts: J23109 + J23032, I712074 + J23032, K124015 + B0015, R0084 + J23022, R0062 + B0032 and C0012+ B0015. They seemed to have ligated properly. After that, we digested these ligations for the next step in the ligation process.
- We MiniPrepped the following parts: C0040 + B0015, C0060 + B0015 and J23100 + B0032. They will be tested tomorrow.
- We continued to make the T7 polymerase wit UmuD tag. Therefore we used PCR in two steps. The first step worked, but the second one didn't. There might be something wrong with the primer (it was a very long one).
