Team:KULeuven/14 August 2008

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Contents

Lab Work

Wet Lab

  • The PCR sampeles of the T7 polymerase with UmuD tag were put on gel. The results were not good.
  • Some more ligation products were cut: J23109 + J23032, E0022 + B0015, C0062 + B0015 with XbaI and EcoRI; R0084 + J23022, R0062 + B0032, J23100 + B0032 with SpeI and EcoRI. Only R0062 + B0032 and J23100 + B0032 were not properly cut. It is possible thet the ligations are not correct.
  • Ligations that failed were set up again: C0040 + B0015, GFP + B0015, C0012 + B0015, C0060 + B0015.

Dry Lab

Modeling

Nick transferred all his assets to us today. He delegated heavily and provided us with workable stuff for next week :) The wiki was also updated and remarks are all around, so a lot of work still to do

Today, we implemented auto-activation of the Lux repressor, mimicking the original Vibrio Lux system. After a lot of messing about and a lot of long kinetic laws we finally managed it and it seems to be working correctly. It's very hard though to keep track of all the correct/changed parameters in such a large system :/

It's been said before this week, but things are looking quite good. We might have reached some sort of end point here where everything should be final. (hopefully)

Anyhow, time for a long weekend. See you on monday!

Wiki

Homepage code reformatted, and content added. Maarten made a image map of the team picture. IE fixes for tabs and picture gallery added.

Remarks