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Team:KULeuven/15 July 2008
From 2008.igem.org
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=== Wetlab === | === Wetlab === | ||
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| + | Today was our first day in the lab. We prepared LB-medium, Ampicilline,... Everything is now ready for the serious labwork: from tips to cleaning our benches. | ||
| + | We did a first attempt to punch out a part of the registry. Then we transformed competent cells (DH5alpha) with the part. We started with the input device: M30109. | ||
| + | Stefanie was very excited to pour plates! | ||
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=== Dry lab === | === Dry lab === | ||
Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of the active site of the enzyme. We'll have to do N-terminal tagging, this could work, see the crystal structure of T7. See the link under literature on this wiki. Lon-mediated degradation of lambda excisionase (Xis) and E.coli UmuD gene product. 30-40 amino temrinal AA of UmuD should do the trick. | Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of the active site of the enzyme. We'll have to do N-terminal tagging, this could work, see the crystal structure of T7. See the link under literature on this wiki. Lon-mediated degradation of lambda excisionase (Xis) and E.coli UmuD gene product. 30-40 amino temrinal AA of UmuD should do the trick. | ||
Revision as of 15:29, 15 July 2008
Contents |
Lab Work
Wetlab
Today was our first day in the lab. We prepared LB-medium, Ampicilline,... Everything is now ready for the serious labwork: from tips to cleaning our benches. We did a first attempt to punch out a part of the registry. Then we transformed competent cells (DH5alpha) with the part. We started with the input device: M30109. Stefanie was very excited to pour plates!
Dry lab
Discovered that the LVA tagging T7 RNA polymerase is a no go as the C-terminus is part of the active site of the enzyme. We'll have to do N-terminal tagging, this could work, see the crystal structure of T7. See the link under literature on this wiki. Lon-mediated degradation of lambda excisionase (Xis) and E.coli UmuD gene product. 30-40 amino temrinal AA of UmuD should do the trick.
Modeling
Parameters, parameters, parameters, ...
Remarks
Project was checked by the presentation audience
