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Team:KULeuven/16 July 2008
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* Yesterday's transformation gave no colonies. | * Yesterday's transformation gave no colonies. | ||
* Today, we performed the same transformation ('''BBa_M30109''') in competent TOP10 cells using the BioBrick protocol (and not the CMPG protocol). An empty pUC plasmid was used as control. | * Today, we performed the same transformation ('''BBa_M30109''') in competent TOP10 cells using the BioBrick protocol (and not the CMPG protocol). An empty pUC plasmid was used as control. | ||
| - | * '''BBa_B0034''' was transformed | + | * '''BBa_B0034''' was transformed competent DH5alpha cells using the CMPG protocol, also with empty pUC control. |
* Buffer CCMB80, SOB agar and SOB broth were made. | * Buffer CCMB80, SOB agar and SOB broth were made. | ||
* Incompetent cells were streaked on a SOB agar, first step in making them competent, following the Registry's protocol. | * Incompetent cells were streaked on a SOB agar, first step in making them competent, following the Registry's protocol. | ||
| - | Because the transformation didn't succeed yesterday, we are a bit concerned about the use of M30109. We found that no project before | + | Because the transformation didn't succeed yesterday, we are a bit concerned about the use of M30109. We found that no project before could ever succesfully use this part. |
=== Dry Lab === | === Dry Lab === | ||
Revision as of 22:25, 17 July 2008
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Contents |
Lab Work
Wet Lab
- Yesterday's transformation gave no colonies.
- Today, we performed the same transformation (BBa_M30109) in competent TOP10 cells using the BioBrick protocol (and not the CMPG protocol). An empty pUC plasmid was used as control.
- BBa_B0034 was transformed competent DH5alpha cells using the CMPG protocol, also with empty pUC control.
- Buffer CCMB80, SOB agar and SOB broth were made.
- Incompetent cells were streaked on a SOB agar, first step in making them competent, following the Registry's protocol.
Because the transformation didn't succeed yesterday, we are a bit concerned about the use of M30109. We found that no project before could ever succesfully use this part.
Dry Lab
Research concerning the UmuD tag we need for the T7 polymerase. UmuD should reduce the half-life of T7 polymerase to approximately 9 minutes. The sequence in FASTA-format and more information about the UmuD tag, is listed under 'Literature'.
Modeling
Parameters... Making very significant progress.
