Team:KULeuven/28 July 2008

From 2008.igem.org

(Difference between revisions)
(Lab Work)
(Modeling)
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== Modeling ==
== Modeling ==
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Great news: the first cycle of modeling is completely finished and everything works as presumed!
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We retrieved some parameters for the cell death-mechanism (thanks to Tokyo 2007) and this worked out just fine. Dries finished his new memory piece and we integrated this into the latest partial model. (The results of the simulation can be found in the modeling section.)
== Remarks ==
== Remarks ==

Revision as of 08:55, 29 July 2008

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Contents

Lab Work

Wet Lab

Today we tried to cut I714891 again, but it didn't work. There can be something wrong with the prefix and the suffix. This is also what's on the quality control page of the registry. Alternatively, we can use the GFP with LVA-tag (present in the lab), but we need primers to attach the prefix and the suffix.

The fragments that ligated during the weekend were transformed in competent TOP10 cells using the iGEM protocol and the CMPG protocol.

The parts for the filter and the inverted were inoculated.

Dry Lab

Modeling

Great news: the first cycle of modeling is completely finished and everything works as presumed! We retrieved some parameters for the cell death-mechanism (thanks to Tokyo 2007) and this worked out just fine. Dries finished his new memory piece and we integrated this into the latest partial model. (The results of the simulation can be found in the modeling section.)

Remarks