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Team:KULeuven/28 July 2008
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Lab Work
Wet Lab
Today we tried to cut I714891 again, but it didn't work. There can be something wrong with the prefix and the suffix. This is also what's on the quality control page of the registry. Alternatively, we can use the GFP with LVA-tag (present in the lab), but we need primers to attach the prefix and the suffix.
The fragments that ligated during the weekend were transformed in competent TOP10 cells using the iGEM protocol and the CMPG protocol.
The parts for the filter and the inverted were inoculated.
