http://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&feed=atom&action=historyTeam:KULeuven/5 September 2008 - Revision history2024-03-29T12:17:14ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=100257&oldid=prevBNathalie at 01:35, 30 October 20082008-10-30T01:35:58Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Homepage has been revamped, removing a lot of bugs. IE fixes still need to follow. Components bar has been fixed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Homepage has been revamped, removing a lot of bugs. IE fixes still need to follow. Components bar has been fixed.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== Remarks ==</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== Strip of the day ==</del></div></td><td colspan="2"> </td></tr>
</table>BNathaliehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=100237&oldid=prevBNathalie at 01:35, 30 October 20082008-10-30T01:35:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We had a very happy day in the lab today and this is what we did:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We had a very happy day in the lab today and this is what we did:</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* We put the digests of R0011+B0033, K145001+B0015, K145150 and K145013 on gel. They seemed OK and so we extracted them out of the gel.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* We put the digests of <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0011 </ins>R0011<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0033 </ins>B0033<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145001 </ins>K145001<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145150 </ins>K145150<ins class="diffchange diffchange-inline">] </ins>and <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145013 </ins>K145013<ins class="diffchange diffchange-inline">] </ins>on gel. They seemed OK and so we extracted them out of the gel.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* We made a <del class="diffchange diffchange-inline">Miniprep </del>and digest of the following parts: <div style="margin-left:10px;"> cut with ''Eco''RI and ''Spe''I -> <del class="diffchange diffchange-inline">R0053</del>+<del class="diffchange diffchange-inline">P0152</del>, <del class="diffchange diffchange-inline">R0040</del>+B0033, <del class="diffchange diffchange-inline">R0040</del>+<del class="diffchange diffchange-inline">J23066</del>, <del class="diffchange diffchange-inline">B0014</del>+<del class="diffchange diffchange-inline">B0033 </del>and R1052. </div> <div style="margin-left:10px;"> cut with XbaI -> R0040+E0240, <del class="diffchange diffchange-inline">C0061</del>+B0015 and <del class="diffchange diffchange-inline">C0056</del>+B0015. </div></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* We made a <ins class="diffchange diffchange-inline">miniprep </ins>and digest of the following parts: <div style="margin-left:10px;"> cut with ''Eco''RI and ''Spe''I -> <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0040 R0040]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0033 B0033]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0014 B0014]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0033 </ins>B0033<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0053 R0053]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_P0152 P0152]</ins>, <ins class="diffchange diffchange-inline"> [http://partsregistry.org/Part:BBa_R0040 R0040]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_J23066 J23066] </ins>and <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R1052 </ins>R1052<ins class="diffchange diffchange-inline">]</ins>. </div> <div style="margin-left:10px;"> cut with XbaI -> <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0040 </ins>R0040<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_E0240 </ins>E0240<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_C0056 C0056]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">] </ins>and <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_C0061 C0061]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">]</ins>. </div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* We made a glycerolstock of the ligations that have suceeded so far <del class="diffchange diffchange-inline">(</del>C0040+<del class="diffchange diffchange-inline">B0014</del>, C0060+B0015, C0012+B0015, K145015+B0015, <del class="diffchange diffchange-inline">K145151+B0015 and </del>R0040+E0240<del class="diffchange diffchange-inline">)</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* We made a glycerolstock of the ligations that have suceeded so far <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_C0040 </ins>C0040<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 B0015]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_C0060 </ins>C0060<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_C0012 </ins>C0012<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145015 </ins>K145015<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0015 </ins>B0015<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_R0040 </ins>R0040<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_E0240 </ins>E0240<ins class="diffchange diffchange-inline">], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* We set up the following ligations: K145150+pSB1A2, K145150+E0240, K145150+B0034, K145013+pSB1A2 and (B0014+B0033)+(K145151+B0015).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* We set up the following ligations: <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145150 </ins>K145150<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:</ins>pSB1A2 <ins class="diffchange diffchange-inline">pSB1A2]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145150 </ins>K145150<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_E0240 </ins>E0240<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145150 </ins>K145150<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_B0034 </ins>B0034<ins class="diffchange diffchange-inline">]</ins>, <ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:BBa_K145013 </ins>K145013<ins class="diffchange diffchange-inline">]</ins>+<ins class="diffchange diffchange-inline">[http://partsregistry.org/Part:pSB1A2 </ins>pSB1A2<ins class="diffchange diffchange-inline">] </ins>and (B0014+B0033)+(K145151+B0015).</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Unfortunately, there were also some failures. The PCR for T7 polymerase with UmuD tag failed again. The result of the PCR test of the transduction was also a bit dodgy and the digest of R1052 resulted in a smear (but apparently it is a crappy part). A final attempt to construct T7 polymerase with UmuD tag was started (PCR).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Unfortunately, there were also some failures. The PCR for T7 polymerase with UmuD tag failed again. The result of the PCR test of the transduction was also a bit dodgy and the digest of R1052 resulted in a smear (but apparently it is a crappy part). A final attempt to construct T7 polymerase with UmuD tag was started (PCR).</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Final <del class="diffchange diffchange-inline">Scheme </del>so far:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Final <ins class="diffchange diffchange-inline">Schemes </ins>so far:</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:5sept final.PNG|<del class="diffchange diffchange-inline">center</del>|<del class="diffchange diffchange-inline">700px</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:5sept final.PNG|<ins class="diffchange diffchange-inline">left</ins>|<ins class="diffchange diffchange-inline">thumb|370px]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Image:5sept parallel.PNG|right|thumb|385px</ins>]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">Parallel Scheme so far:</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[Image:5sept parallel.PNG|center|700px]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">NOT THAT MUCH MORE PARTS TO FINISH!!!!</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td></tr>
</table>BNathaliehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=81116&oldid=prevElkevanassche at 19:15, 28 October 20082008-10-28T19:15:24Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:15, 28 October 2008</td>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:KULeuven/Tools/Styling}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:KULeuven/Tools/Header}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:KULeuven/Tools/Header}}</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:KULeuven/Tools/New_Day/Date_Retriever}}</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Lab Work ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Lab Work ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Strip of the day ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Strip of the day ==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">{{:Team:KULeuven/Tools/New_Day/Date_Retriever}}</del></div></td><td colspan="2"> </td></tr>
</table>Elkevanasschehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42872&oldid=prevBNathalie at 20:04, 5 September 20082008-09-05T20:04:09Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Lab Work ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Lab Work ==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Wet Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Wet Lab ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Succes </del>in the <del class="diffchange diffchange-inline">Wet Lab</del>: we <del class="diffchange diffchange-inline">constructed </del>the <del class="diffchange diffchange-inline">OUTPUT</del>-<del class="diffchange diffchange-inline">subsystem </del>with GFP with and without LVA<del class="diffchange diffchange-inline">-</del>tag, as can be seen in this picture. On the left side GFP with LVA tag (our part K145015): less fluorescent. On the right side GFP without LVA tag (part <del class="diffchange diffchange-inline">E0042</del>): more fluorescent.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We had a very happy day </ins>in the <ins class="diffchange diffchange-inline">lab today and this is what we did</ins>:</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We put the digests of R0011+B0033, K145001+B0015, K145150 and K145013 on gel. They seemed OK and so </ins>we <ins class="diffchange diffchange-inline">extracted them out of </ins>the <ins class="diffchange diffchange-inline">gel.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We made a Miniprep and digest of the following parts: <div style="margin</ins>-<ins class="diffchange diffchange-inline">left:10px;"> cut </ins>with <ins class="diffchange diffchange-inline">''Eco''RI and ''Spe''I -> R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033 and R1052. </div> <div style="margin-left:10px;"> cut with XbaI -> R0040+E0240, C0061+B0015 and C0056+B0015. </div></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We made a glycerolstock of the ligations that have suceeded so far (C0040+B0014, C0060+B0015, C0012+B0015, K145015+B0015, K145151+B0015 and R0040+E0240).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* We set up the following ligations: K145150+pSB1A2, K145150+E0240, K145150+B0034, K145013+pSB1A2 and (B0014+B0033)+(K145151+B0015).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Unfortunately, there were also some failures. The PCR for T7 polymerase with UmuD tag failed again. The result of the PCR test of the transduction was also a bit dodgy and the digest of R1052 resulted in a smear (but apparently it is a crappy part). A final attempt to construct T7 polymerase with UmuD tag was started (PCR).</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">But the most exciting thing today, were our flourescent colonies! We constructed two OUTPUT-subsystems containing </ins>GFP with and without LVA tag, as can be seen in this picture. On the left side GFP with LVA tag (our part K145015): less fluorescent. On the right side GFP without LVA tag (part <ins class="diffchange diffchange-inline">E0240</ins>): more fluorescent. <ins class="diffchange diffchange-inline">This means that our output and GFP-LVA work - hurrah!</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:DSC02957.JPG|center|800px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:DSC02957.JPG|center|800px]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Final Scheme <del class="diffchange diffchange-inline">till </del>so far:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Final Scheme so far:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:5sept final.PNG|center|700px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:5sept final.PNG|center|700px]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Parallel Scheme <del class="diffchange diffchange-inline">till </del>so far:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Parallel Scheme so far:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:5sept parallel.PNG|center|700px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:5sept parallel.PNG|center|700px]]</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 19:</td>
<td colspan="2" class="diff-lineno">Line 29:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>NOT THAT MUCH MORE PARTS TO FINISH!!!!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>NOT THAT MUCH MORE PARTS TO FINISH!!!!</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* We MiniPrepped a few ligations and part R1052.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* We continued the digestion of K145001+B0015 and put R0011+B0032, K145150, K145013 on gel.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* The PCR we did yesterday (transduction and T7) was put on gel, but they both were not correct.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* We tried again to do a PCR of the T7 polymerase with UmuD tag.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td></tr>
</table>BNathaliehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42871&oldid=prevElkevanassche: /* Wet Lab */2008-09-05T19:35:33Z<p><span class="autocomment">Wet Lab</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:35, 5 September 2008</td>
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<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We MiniPrepped a few ligations and part R1052.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We MiniPrepped a few ligations and part R1052.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* We continued the digestion of R0011+ B0032, <del class="diffchange diffchange-inline">K145001+B0015</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* We continued the digestion of <ins class="diffchange diffchange-inline">K145001+B0015 and put </ins>R0011+B0032, <ins class="diffchange diffchange-inline">K145150, K145013 on gel.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* The PCR we did yesterday (transduction and T7) was put on gel, but they both <del class="diffchange diffchange-inline">failed</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* The PCR we did yesterday (transduction and T7) was put on gel, but they both <ins class="diffchange diffchange-inline">were not correct</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We tried again to do a PCR of the T7 polymerase with UmuD tag.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We tried again to do a PCR of the T7 polymerase with UmuD tag.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015</div></td></tr>
</table>Elkevanasschehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42870&oldid=prevElkevanassche: /* Wet Lab */2008-09-05T19:31:15Z<p><span class="autocomment">Wet Lab</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:31, 5 September 2008</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 19:</td>
<td colspan="2" class="diff-lineno">Line 19:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>NOT THAT MUCH MORE PARTS TO FINISH!!!!</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>NOT THAT MUCH MORE PARTS TO FINISH!!!!</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* We MiniPrepped a few ligations and part R1052.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* We continued the digestion of R0011+ B0032, K145001+B0015</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* We digested some more parts (R0053+P0152, R0040+B0033, R0040+J23066, B0014+B0033, R1052 with ''Eco''RI and ''Spe''I; C0061+B0015, C0056+B0015, R0040+E0240 with ''Xba''I). Only R1052 didn't cut well.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* A glycerolstock was made of successful ligations: C0012+B0015, C0060+B0015, C0040+B0015, R0040+E0240 and xxx </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* The PCR we did yesterday (transduction and T7) was put on gel, but they both failed.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* We tried again to do a PCR of the T7 polymerase with UmuD tag.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* We set up some more ligations: K145150+pSB1A2, 145150+E0240, K145150+B0034, K145013+pSB1A2, B0014+B0033+K145151+B0015</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td></tr>
</table>Elkevanasschehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42753&oldid=prevNickvd: /* Wet Lab */2008-09-05T16:15:26Z<p><span class="autocomment">Wet Lab</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 16:15, 5 September 2008</td>
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<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:DSC02957.JPG|center|800px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[Image:DSC02957.JPG|center|800px]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Final Scheme till so far:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:5sept final.PNG|center|700px]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Parallel Scheme till so far:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[Image:5sept parallel.PNG|center|700px]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">NOT THAT MUCH MORE PARTS TO FINISH!!!!</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td></tr>
</table>Nickvdhttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42737&oldid=prevNickvd: /* Modeling */2008-09-05T15:54:23Z<p><span class="autocomment">Modeling</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:54, 5 September 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Modeling ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Modeling ====</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">SimBiology2Latex Toolbox has been finalized and can be found on the wiki.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">MultiCell Toolbox has entered it's final design stages. A preview of the GUI can be found on the wiki.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Some more work on diffusion has been done. Sensitivity Analyses is still a pain in the ***.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wiki ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wiki ====</div></td></tr>
</table>Nickvdhttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42718&oldid=prevHanne at 15:36, 5 September 20082008-09-05T15:36:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Dry Lab ===</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Dr. Coli and his danger, ethics, ethics, ethics...</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Modeling ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Modeling ====</div></td></tr>
</table>Hannehttp://2008.igem.org/wiki/index.php?title=Team:KULeuven/5_September_2008&diff=42706&oldid=prevAvdmeers: /* Wiki */2008-09-05T15:30:49Z<p><span class="autocomment">Wiki</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:30, 5 September 2008</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wiki ====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==== Wiki ====</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Homepage has been revamped, removing a lot of bugs. IE fixes still need to follow. Components bar has been fixed.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Remarks ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Remarks ==</div></td></tr>
</table>Avdmeers