Team:KULeuven/7 August 2008

From 2008.igem.org

(Difference between revisions)
(Dry Lab)
(Dry Lab)
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Cell death contains an error: P1010 is a part which '''constitutively''' produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information].
Cell death contains an error: P1010 is a part which '''constitutively''' produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information].
Primers will have to be made to create a full-blown biobrick with just the ccd locus.
Primers will have to be made to create a full-blown biobrick with just the ccd locus.
 +
A new part has been made from P1010 with just the ccdB coding sequence. We will PCR this part starting from P1010 with the correct primers.
== Modeling ==
== Modeling ==

Revision as of 14:55, 7 August 2008

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< previous friday ← yesterday tomorrow → next monday >

Contents

Lab Work

Dr. Coli overview (for now)

Wet Lab

  • F1610 wasn't cut with XbaI. We are doing this again. The result will follow tomorrow.
  • The primers finally arrived and we are doing a PCR on the ligated parts.
  • We'll do some ligations today: C0012 + B0015, R0062+B0032, R0084+J23022.

Dry Lab

Cell death contains an error: P1010 is a part which constitutively produces ccdB. It already contains a promoter and a RBS, see the [http://partsregistry.org/Part:BBa_P1010 parts page] and the [http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20P1010 Hard Information]. Primers will have to be made to create a full-blown biobrick with just the ccd locus. A new part has been made from P1010 with just the ccdB coding sequence. We will PCR this part starting from P1010 with the correct primers.

Modeling

Wiki

Remarks