Team:KULeuven/Model/Filter

(Difference between revisions)

Revision as of 10:21, 14 August 2008

dock/undock dropdown

*Parameter values Filter*
Name	Value	Comments	Reference
Degradation rates
d_{pT7_tag}	0.00155 s^-1	tag added to speed up degradation of otherwise too stable T7 polymerase	link
d_{mRNA_RIBOKEY}	0.00462 s^-1		link
d_{closed_mRNA_T7}	0.00462 s^-1		link
d_{open_mRNA_T7}	0.00231 s^-1		link
d_{open_mRNA_T7_complex}	0.00231 s^-1		link
Equilibrium constants
K_{eq_1}	0,015 [M]	closed and open mRNA
K_{eq_2}	0.0212 [M]	closed and key (binded with ribokey) complex mRNA
Transcription rates
k_{mRNA_RIBOKEY}	Input dependent
k_{mRNA_T7}	0,0011 s^-1

Remark: The key-lock system can be enhanced to 0.3%-14% (todo: Jonas/Dries)

Matlab diagram for the filter system:

Discussion of the simulation:

1. AND gate of the filter

 ribokey --> T7 --> |
   |                | --> lactonase
   |--------------> |

2. Filtering in practice

First Lightpulse: 300s
- small peak of lactonase
- no influence on the timer
Second Lightpulse: 1000s
- medium peak of lactonase
- influences the timer by levelling the timing capabilities, but it doesn't reset the timer
Third Lightpulse: 5000s
- huge peak of lactonase
- reset of the timer: amount of complex goes to zero

@@ Line 103: / Line 103: @@
 [[Image:Sim_filter_1.png|800px|center|filter]]
+Discussion of the simulation:
+. AND gate of the filter
+  ribokey --> T7 --> |
+    |                | --> lactonase
+    |--------------> |
+. Filtering in practice
+*First Lightpulse: 300s
+**small peak of lactonase
+**no influence on the timer
+*Second Lightpulse: 1000s
+**medium peak of lactonase
+**influences the timer by levelling the timing capabilities, but it doesn't reset the timer
+*Third Lightpulse: 5000s
+**huge peak of lactonase
+**reset of the timer: amount of complex goes to zero
 === Sensitivity Analysis ===
 [[Image:Sens Filter.png|center]]