Team:KULeuven/Model/Inverter

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==== Matlab ([https://static.igem.org/mediawiki/2008/0/00/Inverter_Matlab.zip SBML file])====
==== Matlab ([https://static.igem.org/mediawiki/2008/0/00/Inverter_Matlab.zip SBML file])====
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[[Image:Inverter_Matlab.jpg|400px|center|Inverter]]
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[[Image:Inverter_Matlab.jpg|700px|center|Inverter]]
=== Simulations ===
=== Simulations ===

Revision as of 16:41, 30 August 2008

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Pictogram inverter.png

Contents

Invertimer

Position in the system

The invertimer subsystem receives its input from the filter, T7. The invertimer's function is to produce HSL when no input is present, so a low T7 input gives rise to a high HSL output and vice versa. The production of HSL means that the cell will start a timer that eventually will be used in the celldeath-subsystem to produce ccdB. In this way the cell will die off if no desease remains present.

Describing the system

see also: Project:Invertimer

Inverter BioBrick.jpg

ODE's

Parameters

Parameter values (Inverter)
Name Value Comments Reference
Degradation Rates
dLuxI dLVA = 2.814E-4 s-1 LVA-tag reduces lifetime to 40 minutes link link
dRNA_LuxI 0.0025 s-1 link
dLuxI_antimRNA 0.0045303737 s-1 estimate: because this RNA isn't translated, it degrades faster
dLacI dLVA = 2.814E-4 s-1 LVA-tag reduces lifetime to 40 minutes link link
dclosed mRNA LacI 0.0046209812 s-1 estimate: because this mRNA isn't translated, it degrades faster
dopen mRNA LacI 0.0023104906 s-1 link
dopen mRNA LacI complex 0.0023104906 s-1 link
dHSL 1.02E-6 s-1 very stable in the medium, lifetime around 185h link
LuxI catalysis
kcat 0.0166666667 s-1 Estimated to be about 90% of Vmax in LB medium. link
T7 Transcription
KT7 421 dissociation constant, recalculated to remove units link
kmax 0.044 s-1 maximal T7 transcription rate link
Key-Lock constants
Keq 1 0,015 [M] between closed and open T7 mRNA, experimental link
Keq 2 0.0212 [M] between closed T7 mRNA and key unlocked mRNA complex, experimental link
kdis2 0.00416 s-1 derived from experimental values link
kcomplex2 0.00237 s-1 derived from experimental values link
kclosed 500 s-1 derived from experimental values link
kopen 7.5 s-1 derived from experimental values link
LacI repression
KLacI 1.0E-10 M-1 Dissociation constant
nLacI 2.0 Hill coefficient for LacI link
k_trans_LacI 0.0025 s-1 Estimated maximal transcription rate from R0011 link
Antisense LuxI
k_complex3 0.00237 s-1 rate constant for formation of asRNA - LuxI mRNA duplex
KmRNA_LuxI:antisense_mRNA 4.22E14 Complex of LuxI mRNA with antisense mRNA
Translation Rates
ktransl LuxI 0.167 s-1 translation rate for B0032 RBS (0.3 relative efficiency) link
ktransl LacI 0.167 s-1 lock defined translation rate for LacI

Models

CellDesigner (SBML file)

Inverter

Matlab (SBML file)

Inverter

Simulations

Remark: simulation is not from latest version.

filter

Sensitivity Analysis

Sens Inverter.png