"
Team:KULeuven/Project/Inverter
From 2008.igem.org
m |
|||
| Line 4: | Line 4: | ||
==Inverter== | ==Inverter== | ||
| + | ===BioBricks=== | ||
[[Image:Inverter_BioBrick.jpg|center]] | [[Image:Inverter_BioBrick.jpg|center]] | ||
| - | The input signal for the inverter is filtered by using the T7 RNA polymerase promoter '''BBa_I712074''' and RiboLock3d '''BBa_J23032'''. The inverter's output signal is the LuxI protein, encoded by '''BBa_C0061'''. (''' | + | ===Components=== |
| + | The input signal for the inverter is filtered by using the T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) and RiboLock3d ([http://partsregistry.org/Part:BBa_J23032 '''BBa_J23032''']). The inverter's output signal is the LuxI protein, encoded by [http://partsregistry.org/Part:BBa_C061 '''BBa_C0061''']. On the basis of the inverter system is the LacI repressor molecule, encoded by [http://partsregistry.org/Part:BBa_C0012 '''BBa_C0012'''], and the lac promotor ([http://partsregistry.org/Part:BBa_R0011 '''BBa_R0011''']). | ||
| - | + | ||
| + | ===Action=== | ||
| + | When there is a consistent input signal from the filter system, LacI is produced. This part ([http://partsregistry.org/Part:BBa_C0012 '''BBa_C0012''']) contains a C-terminal [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for faster degradation. LacI inhibits transcription from the lac promoter, and this inhibition can be prevented by binding of LacI to IPTG. When no (or not enough, because of the [https://2008.igem.org/Team:KULeuven/Project/Filter filter]) signal is present, the LacI repressor will not be made and LuxI will be produced. It is thus clear that when the filter system does produces an input signal for the inverter, there will be repression of LuxI production. Otherwise, when no input signal from the filter system is present, LuxI will be produced. | ||
Revision as of 10:13, 31 July 2008
Contents |
Inverter
BioBricks
Components
The input signal for the inverter is filtered by using the T7 RNA polymerase promoter (BBa_I712074) and RiboLock3d (BBa_J23032). The inverter's output signal is the LuxI protein, encoded by BBa_C0061. On the basis of the inverter system is the LacI repressor molecule, encoded by BBa_C0012, and the lac promotor (BBa_R0011).
Action
When there is a consistent input signal from the filter system, LacI is produced. This part (BBa_C0012) contains a C-terminal LVA tag for faster degradation. LacI inhibits transcription from the lac promoter, and this inhibition can be prevented by binding of LacI to IPTG. When no (or not enough, because of the filter) signal is present, the LacI repressor will not be made and LuxI will be produced. It is thus clear that when the filter system does produces an input signal for the inverter, there will be repression of LuxI production. Otherwise, when no input signal from the filter system is present, LuxI will be produced.
