Team:KULeuven/Project/Output

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Revision as of 12:22, 25 August 2008

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Output

We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.

BioBricks

Components

A GFP with a C-terminal LVA tag for rapid degradation of the protein (Anderson et al, 1998) was chosen so that we can follow the output signal quickly in time. The OmpF promoter (BBa_R0084) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen BBa_B0032 with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.

References

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842

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 ===Components===
-A GFP with a C-terminal [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein (Anderson et al, 1998), was chosen so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
+A GFP with a C-terminal [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein (Anderson et al, 1998) was chosen so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
 ===Action===