Team:KULeuven/Project/Reset

From 2008.igem.org

(Difference between revisions)

Revision as of 16:00, 10 September 2008

dock/undock dropdown

Reset

BioBricks

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter (BBa_I712074) followed by a RiboLock (BBa_J23078). The aiiA gene (BBa_C0060), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. (reference)

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.

Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.

@@ Line 15: / Line 15: @@
 This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter Timer].
-When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
+When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.
-Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised that is described here. Now the the amount of lactonase produced should be high enough for the reset to function properly.
+Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.
 {{:Team:KULeuven/Tools/Components}}