Team:Lethbridge CCS/Notebook

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Latest revision as of 00:34, 19 October 2008

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July & August Notebook

September Notebook

October Notebook

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 <!-- {{#calendar: title=Lethbridge_CCS |year=2008 | month=05}} -->
+[[Image:Lethbridge_CCS_logo.png|Logo|100px]]     [[Team:Lethbridge_CCS|Team Home]]
-=== 2 Sept 2008 ===
+<!-- === July &amp; August === -->
-(Peter, Paul, Elizabeth, Glenda, Nathan)
+'''[[Team:Lethbridge_CCS/Notebook/July &amp; August| July &amp; August Notebook]]'''
-* Resuspended our primers (psB1A7 forward and reverse; TetR-GFP forward and reverse) to 100 &mu;M.
-* Made 10-fold dilutions of each primer
-* Set up and ran PCR on psB1A7
-** varied concentration of vector (1 x, 1/10 x, 1/100 x) and annealing temperatures (58&deg;C, 62&deg;C, 66&deg;C), giving nine variations;
-** used materials and amounts as per Phusion polymerase kit
- - Cycling temperatures and times for PCR
+'''[[Team:Lethbridge_CCS/Notebook/September|September Notebook]]'''
-     Initial denaturation           98&deg;C            30 sec   ___
-     Denaturation                   98&deg;C            10 sec      |
-     Annealing                      58/62/66 &deg;C     30 sec      |--- 30 cycles
-     Extension                      72&deg;C            1 min    ___|
-     Final extension                72&deg;C            10 min
-     Hold                           4&deg;C             hold
-<!-- [[Image:pic, if needed]] -->
+'''[[Team:Lethbridge_CCS/Notebook/October|October Notebook]]'''
-=== 6 Sept 2008 ===
-* Agarose gel purification of pSB1A7 from [date] (Peter, Paul, Marc, Glenda, Nathan)
- - conditions
-<!-- [[Image:pic, if needed]] -->
+<div style="display:block; text-align:center; border:2px solid #6C5; width:500px; margin:auto; margin-top:3em; padding:5px;">
+  <p> [[Team:Lethbridge_CCS | Main Page]] &#124; [[Team:Lethbridge_CCS/Team | Team ]] &#124; [[Team:Lethbridge_CCS/Project | Project ]] &#124; [[Team:Lethbridge_CCS/Notebook | Lab Notebook]] </p>
+</div>
 <!-- ==Notebook==