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Team:MIT/Colony PCR
From 2008.igem.org
(Difference between revisions)
(New page: Colony PCR 2 uL primer #1 (to 10uM) 2 uL primer #2 0.4 uL DNTPs 2 uL Buffer 5 uL colony culture 0.2 uL Taq 10.4 uL H20 92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00) |
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2 uL primer #1 (to 10uM) | 2 uL primer #1 (to 10uM) | ||
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2 uL primer #2 | 2 uL primer #2 | ||
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0.4 uL DNTPs | 0.4 uL DNTPs | ||
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2 uL Buffer | 2 uL Buffer | ||
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5 uL colony culture | 5 uL colony culture | ||
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0.2 uL Taq | 0.2 uL Taq | ||
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10.4 uL H20 | 10.4 uL H20 | ||
Revision as of 20:12, 15 August 2008
Colony PCR
2 uL primer #1 (to 10uM)
2 uL primer #2
0.4 uL DNTPs
2 uL Buffer
5 uL colony culture
0.2 uL Taq
10.4 uL H20
92C 1:30 (92 0:30 50 :30 72 1:30) repeat x30 72 10:00
