Team:NTU-Singapore/Modelling/Deterministic Modeling

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Contents

Results of Modeling

Lactose Induced GFP production

GFP Production Model


E7 production system

E7 Production Model E7 Production Modeling Results

Modeling of the System shows that Lactose induction is essential to produce E7 and a variation of Lactose input can result in different yields of E7

Lysis Production system

Lysis Production Model Lysis Prodcution Modeling Results

Here we define 1 as a certain threshold e.g. (<250 µM) that when the lysis protein reaches it, lysis in the cell definitely occurs. Although we wish for an absolute AND gate, where 0 will have no lysis production at all, simulation on biological systems shows that such results are impossible. Both addition Fe ions and Ai-2 alone would induce a certain level of lysis production. However when both are available, the lysis protein production would be higher.


In the presence of Ai-2 alone, Lysis will most likely still occur albeit at a slower rate compared to situation when both Fe and Ai-2 are present.

With the addition of iron and Ai-2 together the rate of lysis production is still significantly much higher compared to Ai-2 alone.

Therefore, by giving the logical output ‘1’ as a suitable threshold value of Lysis protein production higher than that of Ai-2 induction alone, we would still be able to obtain an AND gate based on our definition.