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Team:NYMU-Taipei/Project/Attachment
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(New page: == Motivation == *E. coli is a bacterium that commonly lives in the intestines of people and animals. == Goal == *When genetic E.coli sense the alternation of pH in intestine, adhesion me...) |
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== Motivation == | == Motivation == | ||
| - | *E. coli is a bacterium that commonly lives in the intestines of people and animals. | + | *''E. coli'' is a bacterium that commonly lives in the intestines of people and animals. |
== Goal == | == Goal == | ||
| - | *When genetic E.coli | + | *When genetic ''E.coli'' senses the alternation of pH in intestine, the adhesion mechanism will be turned on in order to '''<font color="red">enhance</font>''' the ability of attachment. |
| - | * | + | *The enhanced adhesion will make a chance for genetic ''E.coli'' to have more time to do things that has been designed by the "Clearance of [[Team:NYMU-Taipei/Project/Urea|Urea]], [[Team:NYMU-Taipei/Project/Phosphate|Phosphate]], and [[Team:NYMU-Taipei/Project/Guanidine|Guanidine]]" groups. |
| - | *To cooperate with "Time Regulation" group | + | *To cooperate with the "[[Team:NYMU-Taipei/Project/Time Regulation|Time Regulation]]" group to allow the genetic ''E.coli'' to detach from intestinal epithelia cells after a specified amount of time. |
| - | == How to attach in intestine== | + | == How to attach in intestine == |
| - | *We have two strategies to enhance attachment in intestine: Expression of | + | *We have two strategies to enhance attachment in intestine: Expression of membrane form of <font color="red">'''FimH'''</font> and <font color="red">'''FliC'''</font>. |
[[Image:Attachment_pic.jpg]] | [[Image:Attachment_pic.jpg]] | ||
*We will use the biobrick part! | *We will use the biobrick part! | ||
| - | ** | + | **{{:Team:NYMU-Taipei/Part|BBa_J04500}}. a composite part of a lac promoter (R0010) and a strong ribosome binding site (B0034). |
| - | ** | + | **{{:Team:NYMU-Taipei/Part|BBa_J36835}}. Lpp, the lipoprotein signal peptide. |
| - | + | **{{:Team:NYMU-Taipei/Part|BBa_J36837}} or {{:Team:NYMU-Taipei/Part|BBa_J36838}}. OmpA, one (O1) or five (O5) transmembrane domains, respectively. Both have been shown to work (Earhart, 2000). | |
| - | + | **{{:Team:NYMU-Taipei/Part|BBa_J36841}} or {{:Team:NYMU-Taipei/Part|BBa_J36843}}. Streptavidin, either wild-type "SW" (Howarth, 2006), or single-chain dimeric "SD" (Aslan, 2005). | |
| - | ==What is '''<font color="blue">FimH</font> | + | ==What is '''<font color="blue">FimH</font>?'''== |
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*The FimH protein is the receptor recognizing element of type 1 fimbriae | *The FimH protein is the receptor recognizing element of type 1 fimbriae | ||
*Fimbriae | *Fimbriae | ||
| - | **Function: attachment of bacteria to its host as in case of pathogenic bacteria salmonella typhimurium , Nisseria gonorrhoea , bordella pertussis | + | **Function: attachment of bacteria to its host as in case of pathogenic bacteria ''salmonella typhimurium'', ''Nisseria gonorrhoea'', ''bordella pertussis''. |
| - | **Size and number: Found | + | **Size and number: Found many in number (up to 1000 per cell) having 3 to 25 nm in diameter and 0.5 to 20 micrometer in length. |
| - | * '''Type 1 fimbriae''' are surface organelles of Escherichia coli which mediate '''D-mannose-sensitive binding''' to different host surfaces. | + | * '''Type 1 fimbriae''' are surface organelles of ''Escherichia coli'' which mediate '''D-mannose-sensitive binding''' to different host surfaces. |
* A single type 1 fimbria is a 7-nm-wide and »1-mm-long surface polymer, The bulk of the structure is made up of »1000 copies of the major subunit protein, FimA, polymerized into a right-handed helical structure. | * A single type 1 fimbria is a 7-nm-wide and »1-mm-long surface polymer, The bulk of the structure is made up of »1000 copies of the major subunit protein, FimA, polymerized into a right-handed helical structure. | ||
* Small quantities of the minor components (FimF, FimG, and FimH) are also present. | * Small quantities of the minor components (FimF, FimG, and FimH) are also present. | ||
* The '''FimH''' protein is the '''receptor recognizing element''' of type 1 fimbriae. | * The '''FimH''' protein is the '''receptor recognizing element''' of type 1 fimbriae. | ||
| - | * Studies on Salmonella Typhimurium revealed that FimH adhesin is responsible for bacteria binding to HeLa, HEp-2 and mouse intestinal epithelial cells. | + | * Studies on ''Salmonella Typhimurium'' revealed that FimH adhesin is responsible for bacteria binding to HeLa, HEp-2 and mouse intestinal epithelial cells. |
|- | |- | ||
|} | |} | ||
| - | ==What is '''<font color="blue">FliC</font> | + | ==What is '''<font color="blue">FliC</font>?'''== |
{| | {| | ||
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Revision as of 16:34, 1 August 2008
Contents |
Motivation
- E. coli is a bacterium that commonly lives in the intestines of people and animals.
Goal
- When genetic E.coli senses the alternation of pH in intestine, the adhesion mechanism will be turned on in order to enhance the ability of attachment.
- The enhanced adhesion will make a chance for genetic E.coli to have more time to do things that has been designed by the "Clearance of Urea, Phosphate, and Guanidine" groups.
- To cooperate with the "Time Regulation" group to allow the genetic E.coli to detach from intestinal epithelia cells after a specified amount of time.
How to attach in intestine
- We have two strategies to enhance attachment in intestine: Expression of membrane form of FimH and FliC.
- We will use the biobrick part!
- BBa_J04500. a composite part of a lac promoter (R0010) and a strong ribosome binding site (B0034).
- BBa_J36835. Lpp, the lipoprotein signal peptide.
- BBa_J36837 or BBa_J36838. OmpA, one (O1) or five (O5) transmembrane domains, respectively. Both have been shown to work (Earhart, 2000).
- BBa_J36841 or BBa_J36843. Streptavidin, either wild-type "SW" (Howarth, 2006), or single-chain dimeric "SD" (Aslan, 2005).
What is FimH?
|
What is FliC?
|
Circuit Design
- Preliminary Design
- Design 1File:Attachment.jpg
- Design 2File:Combine 3.jpg
References
- Functional characterization of the FimH adhesin from Salmonella enterica serovar Enteritidis[1]
- Combining sites of bacterial fimbriae [2]
- Probing the receptor recognition site of the FimH adhesin by fimbriae- displayed FimH-FocH hybrids[3]
- Host Protein Binding and Adhesive Properties of H6 and H7 Flagella of Attaching and Effacing Escherichia coli[4]
- Sequence Diversity of the Escherichia coli H7 fliC Genes:Implication for a DNA-Based Typing Scheme forE. coli O157:H7[5]
