Team:NYMU-Taipei/Project/Phosphate

From 2008.igem.org

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* [http://www.ncbi.nlm.nih.gov/pubmed/10448683 Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater, Prog Mol Subcell Biol. 1999]
* [http://www.ncbi.nlm.nih.gov/pubmed/10448683 Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater, Prog Mol Subcell Biol. 1999]
* [http://www.ncbi.nlm.nih.gov/pubmed/12964240 Microbial phosphate removal and polyphosphate production from wastewaters, Adv Appl Microbiol. 2003]
* [http://www.ncbi.nlm.nih.gov/pubmed/12964240 Microbial phosphate removal and polyphosphate production from wastewaters, Adv Appl Microbiol. 2003]
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* [http://www.ncbi.nlm.nih.gov/pubmed/12187377 Enhanced phosphate uptake and polyphosphate accumulation in Burkholderia cepacia grown under low pH conditions, Microb Ecol. 2002] and [[Enhanced phosphate uptake and polyphosphate accumulation in Burkholderia cepacia grown under low pH conditions|abstract]]
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* [http://www.ncbi.nlm.nih.gov/pubmed/12187377 Enhanced phosphate uptake and polyphosphate accumulation in Burkholderia cepacia grown under low pH conditions, Microb Ecol. 2002]
* [http://www.springerlink.com/content/t6qkt5214pqncmjd/ Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface, AMB 2004]
* [http://www.springerlink.com/content/t6qkt5214pqncmjd/ Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface, AMB 2004]
* [http://www.jstage.jst.go.jp/article/jbb/95/6/95_637/_article/-char/en A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering 2005]
* [http://www.jstage.jst.go.jp/article/jbb/95/6/95_637/_article/-char/en A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering 2005]
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Revision as of 08:09, 3 August 2008

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Contents

Motivation

  • When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
  • A high serum concentration of phosphate (pi) is clearly related to pruritus (搔癢症) and hyperparathyroidism (甲狀旁腺功能亢進症), both manifestations of the uremic syndrome.
  • Phosphorus excess also inhibits 1 alpha-hydroxylase and hence the production of calcitriol, the active vitamin D metabolite.
  • Phosphorus retention also alters polyamine metabolism by causing decreases in intestinal function and proliferation of intestinal villi.

Goal

  • Enhance and regulate the phosphate absorption of E.coli to balance phosphate concentration to a normal level in patients with kidney failure.
  • This device operates when:
    • external pH is high.
    • external phosphate level is high.

Circuit Design

NYMU iGEM08 phosphate removal device v 1.0

NYMU IGEM08 phosphate device.png

  • over-expression of pst operon can enhance the total phosphate absorption by improving the cellular phosphate import
  • over-expression of ppK gene can enhance the total phosphate absorption by improving the cellular phosphate storage

References

Phosphate Metabolism and Regulation in E.coli

Phosphate removal by Microbe

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