Team:NYMU-Taipei/Project/Phosphate

From 2008.igem.org

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== Motivation ==
== Motivation ==
* When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
* When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
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* A '''high''' serum concentration of [http://en.wikipedia.org/wiki/Phosphate phosphate (pi)] is clearly related to '''pruritus (搔癢症)''' and '''hyperparathyroidism (甲狀旁腺功能亢進症)''', both manifestations of the uremic syndrome.  
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* A '''high''' serum concentration of [http://en.wikipedia.org/wiki/Phosphate phosphate (pi)] is clearly related to '''pruritus''' and '''hyperparathyroidism''', both manifestations of the uremic syndrome.  
* Phosphorus '''excess''' also '''inhibit'''s 1 alpha-hydroxylase and hence the production of [http://www.nlm.nih.gov/medlineplus/druginfo/medmaster/a682335.html calcitriol], the active vitamin D metabolite.
* Phosphorus '''excess''' also '''inhibit'''s 1 alpha-hydroxylase and hence the production of [http://www.nlm.nih.gov/medlineplus/druginfo/medmaster/a682335.html calcitriol], the active vitamin D metabolite.
* Phosphorus '''retention''' also alters polyamine metabolism by causing decreases in '''intestinal function''' and '''proliferation of intestinal villi'''.
* Phosphorus '''retention''' also alters polyamine metabolism by causing decreases in '''intestinal function''' and '''proliferation of intestinal villi'''.

Revision as of 10:44, 6 August 2008

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Contents

Motivation

  • When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
  • A high serum concentration of phosphate (pi) is clearly related to pruritus and hyperparathyroidism, both manifestations of the uremic syndrome.
  • Phosphorus excess also inhibits 1 alpha-hydroxylase and hence the production of calcitriol, the active vitamin D metabolite.
  • Phosphorus retention also alters polyamine metabolism by causing decreases in intestinal function and proliferation of intestinal villi.

Goal

  • Enhance and regulate the phosphate absorption of E.coli to balance phosphate concentration to a normal level in patients with kidney failure.
  • This device operates when:
    • external pH is high and
    • external phosphate level is high
  • This device stops when
    • external pH is low or
    • external phosphate level is low

Circuit Design

NYMU iGEM08 phosphate removal device v 1.0

NYMU IGEM08 phosphate device.png

  • over-expression of pst operon can enhance the total phosphate absorption by improving the cellular phosphate import
  • over-expression of ppK gene can enhance the total phosphate absorption by improving the cellular phosphate storage

References

Phosphate Metabolism and Regulation in E.coli

Phosphate removal by Microbe

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