Team:NYMU-Taipei/Project/Phosphate

From 2008.igem.org

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(New page: == Motivation == * When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death. * A '''high''' serum concentration of [http://en.wikipedia.org/wi...)
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== Motivation ==
== Motivation ==
* When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
* When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
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== Circuit Design ==
== Circuit Design ==
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NYMU iGEM08 phosphate removal device v 1.0
NYMU iGEM08 phosphate removal device v 1.0
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[[Image:IGEM08 phosphate device.png|600px]]
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[[Image:NYMU_IGEM08_phosphate_device.png|600px]]
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* over-expression of pst operon can enhance the total phosphate absorption by improving the cellular '''phosphate import'''
* over-expression of pst operon can enhance the total phosphate absorption by improving the cellular '''phosphate import'''
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* [http://www.springerlink.com/content/t6qkt5214pqncmjd/ Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface, AMB 2004]
* [http://www.springerlink.com/content/t6qkt5214pqncmjd/ Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface, AMB 2004]
* [http://www.jstage.jst.go.jp/article/jbb/95/6/95_637/_article/-char/en A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering 2005]
* [http://www.jstage.jst.go.jp/article/jbb/95/6/95_637/_article/-char/en A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering 2005]
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Revision as of 17:38, 1 August 2008

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Contents

Motivation

  • When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
  • A high serum concentration of phosphate (pi) is clearly related to pruritus (搔癢症) and hyperparathyroidism (甲狀旁腺功能亢進症), both manifestations of the uremic syndrome.
  • Phosphorus excess also inhibits 1 alpha-hydroxylase and hence the production of calcitriol, the active vitamin D metabolite.
  • Phosphorus retention also alters polyamine metabolism by causing decreases in intestinal function and proliferation of intestinal villi.

Goal

  • Enhance and regulate the phosphate absorption of E.coli to balance phosphate concentration to a normal level in patients with kidney failure.
  • This device operates when:
    • external pH is high.
    • external phosphate level is high.

Circuit Design

NYMU iGEM08 phosphate removal device v 1.0

NYMU IGEM08 phosphate device.png

  • over-expression of pst operon can enhance the total phosphate absorption by improving the cellular phosphate import
  • over-expression of ppK gene can enhance the total phosphate absorption by improving the cellular phosphate storage

References

Phosphate Metabolism and Regulation in E.coli

Phosphate removal by Microbe

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