Team:NYMU-Taipei/Project/Phosphate
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Contents |
Motivation
- When the phosphate level in blood exceeds 6.5 mg/dl, a patient kidney failure has a high risk of death.
- A high serum concentration of phosphate (pi) is clearly related to pruritus (搔癢症) and hyperparathyroidism (甲狀旁腺功能亢進症), both manifestations of the uremic syndrome.
- Phosphorus excess also inhibits 1 alpha-hydroxylase and hence the production of calcitriol, the active vitamin D metabolite.
- Phosphorus retention also alters polyamine metabolism by causing decreases in intestinal function and proliferation of intestinal villi.
Goal
- Enhance and regulate the phosphate absorption of E.coli to balance phosphate concentration to a normal level in patients with kidney failure.
- This device operates when:
- external pH is high.
- external phosphate level is high.
Circuit Design
NYMU iGEM08 phosphate removal device v 1.0 | |
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References
Phosphate Metabolism and Regulation in E.coli
- Characterization of PitA and PitB from Escherichia coli, JB 2001
- Accumulation of Inorganic Polyphosphate in phoU Mutants of Escherichia coli and Synechocystis sp. Strain PCC6803, AEM 2002
- The phosphate regulon and bacterial virulence: a regulatory network connecting phosphate homeostasis and pathogenesis, FEMS Microbiology Reviews 2008
Phosphate removal by Microbe
- Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater, AEM 1993
- Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater, Prog Mol Subcell Biol. 1999
- Microbial phosphate removal and polyphosphate production from wastewaters, Adv Appl Microbiol. 2003
- Enhanced phosphate uptake and polyphosphate accumulation in Burkholderia cepacia grown under low pH conditions, Microb Ecol. 2002 and abstract
- Intelligent yeast strains with the ability to self-monitor the concentrations of intra- and extracellular phosphate or ammonium ion by emission of fluorescence from the cell surface, AMB 2004
- A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering 2005