Team:NYMU-Taipei/Project/Phosphate/Experiments/Assembly

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Contents

Auto-Regulated Phosphate Absorption Device

Phosphate Absorption Circuit

NYMU 20081007 pi absorption design.png

Phosphate Regulatory Circuit

NYMU 20081007 pi regulation design.png

Operations of Design

NYMU 20081007 pi operations.png

Testing Circuits

Test of pTet and tetR regulatory pair

NYMU 20081007 pi regulation test.png

  • RBS (B0034) + tetR (C0040) + D-term (B0015)
    • S03880 (failed;9/19, 2008)
    • P0440 (succeed; 9/19, 2008)

  • plasmid extraction (9/20)
    • pLac: R0010 (54.2 ng/uL)
    • pTet: R0040 (26.4 ng/uL)
    • RBS: B0032 (80.9 ng/uL)
    • D-term: B0015 (118.4 ng/uL)

  • plasmid extraction (9/23)
    • P0440 (114.62 ng/uL)
  • vector digestion (9/23)
    • pLac: R0010 D. SP (10.87 ng/uL)
    • pTet: R0040 D. SP (5.55 ng/uL)
    • RBS: B0032 D. SP (15.32 ng/uL)

Test of PPK and PST for pi absorption

NYMU 20081007 pi absorption test.png