Team:NYMU-Taipei/Project/Phosphate/PRIMER of pstSCAB-phoU PST operon

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Contents

pst Operon

operon structure

NYMU Pst Operon.png

biobrick cutting sites scanning report

NYMU Pst operon cutting site scanning report.png

  • by serial cloner 1.3
>EG10734 pstS (complement(3909548..3904876) E. coli
atgAAAGTTA TGCGTACCAC CGTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT
TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG
TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT
ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT
GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG
ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG
GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC
ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC
GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG
TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA
GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA
TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA
CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA
ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC
ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA
TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC
CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT
AGCGGTAAGC CGCTGTACTA ATAAAACTCC AGGCCGGGTA CGGTGTTTTA CGCCGCATCC
GGCATTACAA AATGACTTTG TAAACGCGTT TAACTGAAGA GTAACTTATG GCTGCAACCA
AGCCTGCTTT TAACCCACCG GGTAAAAAGG GCGACATAAT TTTCAGCGTG CTGGTAAAAC
TGGCGGCGCT GATTGTGCTA TTGATGTTGG GTGGCATTAT TGTCTCTCTG ATCATCTCCT
CCTGGCCGAG CATTCAGAAA TTTGGTCTGG CTTTCCTATG GACCAAAGAG TGGGATGCAC
CGAACGATAT CTACGGGGCG CTGGTGCCGA TCTACGGTAC GTTGGTGACT TCGTTTATCG
CGCTGCTGAT CGCCGTCCCG GTGAGTTTCG GTATCGCCCT GTTCCTGACT GAGCTTGCGC
CTGGCTGGCT GAAACGCCCG CTGGGTATCG CCATTGAGCT GCTGGCAGCC ATTCCAAGTA
TCGTTTACGG CATGTGGGGC CTGTTTATCT TTGCGCCGCT GTTCGCCGTT TACTTTCAGG
AGCCGGTCGG CAATATCATG TCGAATATCC CGATTGTTGG CGCGCTGTTC TCTGGCCCCG
CATTTGGTAT CGGTATCCTC GCGGCAGGCG TGATCCTCGC CATCATGATT ATTCCGTACA
TTGCGGCGGT AATGCGTGAT GTGTTCGAAC AAACCCCGGT GATGATGAAA GAGTCGGCCT
ACGGTATTGG CTGCACCACC TGGGAAGTTA TCTGGCGTAT CGTTCTTCCG TTCACCAAAA
ATGGTGTTAT CGGCGGCATC ATGCTGGGGC TGGGCCGCGC GCTCGGTGAA ACCATGGCGG
TGACCTTTAT CATCGGTAAC ACCTACCAGC TCGACAGCGC CTCGCTGTAT ATGCCGGGCA
ACAGTATCAC CTCTGCGCTG GCGAACGAAT TTGCGGAAGC GGAATCCGGT CTGCACGTTG
CCGCACTGAT GGAACTGGGC CTGATCCTGT TTGTGATTAC CTTCATCGTC CTCGCCGCAT
CGAAGTTTAT GATTATGCGC CTGGCTAAGA ATGAGGGGGC ACGCTAATGG CTATGGTTGA
AATGCAAACC ACTGCGGCGC TGGCTGAATC TCGCCGCAAA ATGCAGGCGC GTCGCCGCCT
CAAAAACCGT ATTGCGCTGA CGCTCTCGAT GGCGACGATG GCCTTCGGCC TGTTCTGGCT
GATCTGGATT TTAATGTCCA CCATCACTCG CGGTATCGAC GGTATGTCGC TGGCGCTGTT
CACTGAAATG ACGCCGCCGC CCAATACGGA AGGTGGTGGT CTGGCGAACG CTCTGGCGGG
TAGCGGGCTG TTAATTTTGT GGGCCACGGT ATTCGGTACG CCGCTGGGCA TTATGGCGGG
GATTTATCTG GCGGAATATG GTCGTAAATC CTGGCTGGCA GAAGTGATTC GCTTCATTAA
CGACATTCTG CTCTCTGCGC CGTCGATTGT GGTTGGTCTG TTTGTTTACA CCATTGTGGT
GGCGCAGATG GAGCACTTCT CCGGCTGGGC GGGCGTGATT GCCCTGGCGT TGTTGCAGGT
GCCGATTGTT ATCCGCACCA CCGAGAACAT GCTGAAACTG GTGCCGTACA GCCTGCGTGA
AGCGGCTTAT GCGCTGGGTA CACCGAAGTG GAAGATGATC TCTGCGATTA CGCTGAAAGC
GTCGGTGTCC GGGATTATGA CCGGTATCCT GCTGGCGATT GCCCGTATTG CTGGTGAAAC
CGCGCCGCTG CTGTTTACCG CGCTCTCCAA CCAGTTCTGG AGCACGGACA TGATGCAGCC
GATCGCCAAC CTGCCGGTGA CGATCTTTAA GTTTGCGATG AGCCCGTTTG CGGAATGGCA
GCAATTGGCC TGGGCCGGGG TATTGATCAT TACCCTGTGC GTACTGCTGC TGAACATTCT
GGCGCGCGTT GTTTTTGCGA AGAATAAACA CGGTTGATAT TGCTGACACG GTTTTCCCCT
CACCCTAACC CTCTCCCCAG AGGGGCGAGG GGACCGACCG AGCGCCTTTT TGACTCTGTA
CACGGTTAAC ACTTTGCCGG ATGCGGCGTG AACGCCTGAT CCGGCCTACG GTAAGCCTGA
TTAGCGAAGT GCATCAGGCA CGATGAGGAA AAGATTGCAA TGAGTATGGT TGAAACTGCC
CCGAGTAAAA TTCAGGTTCG TAATTTGAAC TTCTACTACG GCAAATTCCA TGCCCTGAAA
AACATCAACC TGGATATCGC TAAAAACCAG GTAACGGCGT TTATCGGGCC GTCCGGCTGC
GGTAAATCGA CGCTGCTGCG TACCTTCAAC AAAATGTTTG AACTGTACCC GGAGCAGCGT
GCGGAAGGTG AAATTCTGCT TGATGGCGAC AACATCCTGA CCAACTCTCA GGATATCGCA
CTGCTGCGTG CGAAAGTGGG CATGGTGTTC CAGAAACCGA CGCCGTTTCC GATGTCCATC
TACGACAACA TCGCTTTTGG CGTTCGTCTG TTTGAGAAGC TCTCCCGTGC CGACATGGAC
GAGCGCGTGC AGTGGGCATT GACCAAAGCC GCATTGTGGA ACGAAACCAA AGATAAATTG
CACCAGAGCG GTTACTCTCT CTCTGGTGGT CAGCAACAGC GTCTGTGTAT TGCGCGTGGT
ATCGCCATTC GCCCGGAAGT GCTGCTGCTC GACGAACCGT GTTCGGCGCT CGACCCTATC
TCTACCGGGC GTATTGAAGA GCTGATCACC GAACTGAAGC AGGATTACAC CGTGGTGATC
GTCACCCACA ACATGCAGCA GGCTGCGCGT TGTTCCGACC ACACGGCGTT TATGTACCTG
GGCGAATTGA TTGAGTTCAG CAACACGGAC GATCTGTTCA CCAAGCCAGC GAAGAAACAA
ACAGAAGACT ACATCACCGG TCGTTACGGT TGATTCAGGA GTGCGTTATG GACAGTCTCA
ATCTTAATAA ACATATTTCC GGCCAGTTCA ACGCCGAACT GGAAAGTATC CGCACGCAGG
TGATGACCAT GGGCGGCATG GTGGAGCAGC AGCTTTCTGA TGCAATCACC GCGATGCATA
ACCAGGACAG CGATCTGGCG AAGCGCGTCA TCGAAGGCGA CAAGAACGTC AACATGATGG
AAGTGGCGAT CGATGAAGCC TGCGTGCGCA TTATCGCCAA ACGTCAGCCG ACGGCGAGCG
ACCTGCGACT GGTTATGGTG ATCAGTAAAA CCATTGCCGA GCTGGAGCGT ATTGGCGACG
TGGCGGACAA AATCTGCCGT ACTGCGCTGG AGAAATTCTC CCAGCAGCAT CAGCCGTTGC
TGGTAAGTCT GGAGTCGCTG GGCCGTCATA CCATCCAGAT GCTGCACGAC GTGCTGGACG
CGTTCGCGCG GATGGACATT GACGAAGCGG TACGTATTTA TCGTGAAGAT AAAAAAGTCG
ATCAGGAATA CGAAGGTATT GTTCGTCAAC TGATGACCTA CATGATGGAA GATTCGCGTA
CCATTCCGAG CGTACTTACT GCGCTGTTCT GCGCGCGTTC TATCGAACGT ATTGGCGACC
GCTGCCAGAA TATTTGTGAG TTTATCTTCT ACTACGTGAA GGGGCAGGAT TTCCGTCACG
TCGGTGGCGA TGAGCTGGAT AAACTGCTGG CGGGGAAAGA TAGCGACAAA TAA
  • A overlap between pstA and pstC

pstS - pst Operon Forward Primer

>EG10734 pstS (complement(3909548..3908508)) E. coli
atgAAAGTTA TGCGTACCAC CGTCGCAACT GTTGTCGCCG CGACCTTATC GATGAGTGCT
TTCTCTGTGT TTGCAGAAGC AAGCCTGACA GGTGCAGGTG CAACCTTCCC TGCGCCGGTG
TATGCCAAAT GGGCTGACAC TTACCAGAAA GAAACCGGTA ATAAAGTTAA CTACCAGGGT
ATCGGTTCTT CCGGTGGCGT AAAACAGATT ATCGCTAATA CCGTTGATTT TGGTGCCTCT
GACGCGCCGC TGTCTGACGA AAAACTGGCT CAGGAAGGTC TGTTCCAGTT CCCGACCGTG
ATTGGCGGCG TGGTGCTGGC GGTTAACATT CCAGGGCTGA AGTCTGGCGA ACTGGTGCTG
GATGGTAAAA CCCTCGGCGA CATCTACCTG GGCAAAATCA AGAAGTGGGA TGATGAAGCC
ATCGCCAAAC TGAATCCGGG TCTGAAACTG CCTTCACAAA ACATTGCTGT AGTACGCCGC
GCAGATGGCT CCGGGACTTC CTTCGTCTTC ACCAGCTACC TGGCGAAAGT GAACGAAGAG
TGGAAAAACA ACGTTGGTAC TGGCTCTACC GTAAAATGGC CGATCGGTCT GGGCGGTAAA
GGTAACGACG GTATCGCCGC GTTCGTTCAG CGTCTGCCGG GTGCAATTGG TTATGTTGAA
TATGCTTACG CGAAGCAGAA CAACCTGGCG TACACCAAAC TGATCTCCGC TGATGGTAAA
CCGGTTAGTC CGACCGAAGA AAACTTCGCT AATGCAGCAA AAGGTGCAGA CTGGAGCAAA
ACCTTCGCTC AGGATCTGAC CAACCAGAAA GGCGAAGATG CATGGCCTAT TACCTCTACC
ACGTTCATTC TGATCCACAA AGATCAGAAG AAACCAGAAC AAGGCACAGA AGTGCTGAAA
TTCTTCGACT GGGCGTACAA AACCGGGGCT AAACAGGCGA ACGACCTGGA TTACGCCAGC
CTGCCGGATA GTGTAGTTGA ACAGGTTCGC GCTGCGTGGA AGACCAATAT TAAAGACAGT
AGCGGTAAGC CGCTGTACTA A
  • Annealing - len:21, Tm:54, GC%:43
  • GC GAATTCGCGGCCGCTTCTAG atgAAAGTTA TGCGTACCAC C
  • Total length - 43 (2 + 20 + 21)

phoU - pst Operon Reverse Primer

>EG10735 phoU (complement(3905601..3904876)) E. coli
atgGACAGTC TCAATCTTAA TAAACATATT TCCGGCCAGT TCAACGCCGA ACTGGAAAGT
ATCCGCACGC AGGTGATGAC CATGGGCGGC ATGGTGGAGC AGCAGCTTTC TGATGCAATC
ACCGCGATGC ATAACCAGGA CAGCGATCTG GCGAAGCGCG TCATCGAAGG CGACAAGAAC
GTCAACATGA TGGAAGTGGC GATCGATGAA GCCTGCGTGC GCATTATCGC CAAACGTCAG
CCGACGGCGA GCGACCTGCG ACTGGTTATG GTGATCAGTA AAACCATTGC CGAGCTGGAG
CGTATTGGCG ACGTGGCGGA CAAAATCTGC CGTACTGCGC TGGAGAAATT CTCCCAGCAG
CATCAGCCGT TGCTGGTAAG TCTGGAGTCG CTGGGCCGTC ATACCATCCA GATGCTGCAC
GACGTGCTGG ACGCGTTCGC GCGGATGGAC ATTGACGAAG CGGTACGTAT TTATCGTGAA
GATAAAAAAG TCGATCAGGA ATACGAAGGT ATTGTTCGTC AACTGATGAC CTACATGATG
GAAGATTCGC GTACCATTCC GAGCGTACTT ACTGCGCTGT TCTGCGCGCG TTCTATCGAA
CGTATTGGCG ACCGCTGCCA GAATATTTGT GAGTTTATCT TCTACTACGT GAAGGGGCAG
GATTTCCGTC ACGTCGGTGG CGATGAGCTG GATAAACTGC TGGCGGGGAA AGATAGCGAC
AAATAA
  • Annealing - len:22, Tm:54, GC%:41
  • GC CTGCAGCGGCCGCTACTAGTA GGGGAA AGATAGCGAC AAATAA
  • Total length - 44 (2 + 20 + 22)