From 2008.igem.org
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Latest revision as of 00:32, 30 October 2008
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- tube 1: 101.3 ng/uL (260/280=1.85)
- tube 2: 72.9 ng/uL (260/280 = 1.90)
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PCR of RpaA and SasA
Gel check
| Comments & Actions
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- front part of RpaA
- back part of RpaA
- front part of SasA
- back part ofRpaA
- positive control
Expected length(bp)
- 389
- 420
- 750
- 472
- 1600
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| successfully pcr back-SasA
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| successfully pcr front-RpaA
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successfully pcr back-RpaA and front-SasA; however they are too weak and we encountered a little bit of trouble doing overlap extension PCR
- action:
- gel extraction
- re-design the primer of FP-in RpaA and RP-in SasA
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Reporting assay
Date
| O.D.600
| GFP expression
| Protocol & Comments
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9/29
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- The M9 does not seems to have enough nutrition for E. coli to grow.
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10/4
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9/30
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| * For the 3 hr measure, the plate was not washed carefully, so there is a large background.
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10/4
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Discussion of the reporting
- Is the freshness of the bacteria important to GFP expression? If so, we should use freshly streaked plates.
- The white and black plates must be cleaned carefully, otherwise there would be too much background.
- pBF seemes to grow much faster than the others.