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From 2008.igem.org

Contents 1 Methodology 2 Calibration of pLac (R0010) 2.1 pLac + E0240 under 0 and 50uM IPTG 2.2 pLac + E0240 under 50, 500 and 1,000uM IPTG 3 References

Methodology

It is an indirect method to quantitatively characterize the coding regions with specific functions, e.g. waste removal efficiency, tuner of oscillator.

Calibration of pLac (R0010)

pLac + E0240 under 0 and 50uM IPTG

pLac + E0240 under 50, 500 and 1,000uM IPTG

References

• gene regulation and operon
• b-Galactosidase Activity Assay
• The pET Expression System
• The lac operon
• The Effect of the lacY Gene on the Induction of IPTG Inducible Promoters, Studied in Escherichia coli and Pseudomonas fluorescens, Current Microbiology 1997
• Combinatorial transcriptional control of the lactose operon of Escherichia coli, PNAS 2007 (Thomas Kuhlman and et. al)

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