Team:Paris/August 24

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Construction for synchronization

Gel extraction

Protocol

Gel 1 (no image sorry, we forgot to save it!)

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample 1kb ladder MP105.1 no sample D107 no sample D109 no sample MP106 no sample D110 no sample 100pb ladder
Expected size (pb) - 2764 - 2750
14
- 2056
708
- 2782 - 2768
14
- -
Measured size (pb) - 2800 - 2750 - 2000
700
- 2800 - 2750 - -

Gel 2

Well 1 2 3 4 5 6 7 8 9 10 11 12
Sample no sample 1kb ladder MP118.1 no sample D125 no sample MP143 no sample D163 no sample 100pb ladder no sample
Expected size (pb) - - 3318 - 3303
15
- 3322 - 2432
890
- - -
Measured size (pb) - - 2900 - 3300 - 2900 - 2500
900
- - -

Ligation

Protocol

Ligation Name Vector Name Volume Vector (µL) Insert Volume Insert (µL)
L159 D125 6 D109 3.86
L162 D107 5 D163 5.3
L163 D110 7.5 D163 5.32
Control L159 D125 6 - -
Control L162 D107 5 - -
Control L163 D110 7.5 - -