Team:Paris/July 14

From 2008.igem.org

(Difference between revisions)
Line 22: Line 22:
* 500ml of SOB ( 2 x 250ml)
* 500ml of SOB ( 2 x 250ml)
* 500ml of SOC (2 x 250ml)
* 500ml of SOC (2 x 250ml)
-
* 100ml of MgCl2 (1M)
+
* 100ml of MgCl<sub>2</sub> (1M)
-
* 150ml of CaCl2 (1M)
+
* 150ml of CaCl<sub>2</sub> (1M)
* 200ml of KOAC (1M)
* 200ml of KOAC (1M)

Revision as of 08:50, 16 July 2008

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Results of the transformation :

  • Positive control : puc19 (1µl; 10pg/ml) --> No transformation
  • Biobricks 2007 : --> succeed of the transformation unlike the delay added in the ice
  • Biobricks 2008 : --> No transformation
          Conclusion : Cells are competents, because there is positive transformation
          Hypothesis : problem with the protocol of DNA extraction from the registery 2008


Culture of Cells :

  • Culture of DH5ɑ in 5ml of LB without Antibiotics


Preparation of medium and solutions :

  • 1L of CCMB80
  • 500ml of SOB ( 2 x 250ml)
  • 500ml of SOC (2 x 250ml)
  • 100ml of MgCl2 (1M)
  • 150ml of CaCl2 (1M)
  • 200ml of KOAC (1M)