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Team:Paris/Modeling/More f1 Table
From 2008.igem.org
(Difference between revisions)
| Line 7: | Line 7: | ||
|comments | |comments | ||
|- | |- | ||
| - | | | + | |ƒ1 |
| - | | | + | |activity of <br> ''pTet'' '''with RBS E0032''' |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
|need for 20 values with well choosen [aTc]<sub>i</sub> <br> | |need for 20 values with well choosen [aTc]<sub>i</sub> <br> | ||
| - | |||
|- | |- | ||
|γ<sub>GFP</sub> | |γ<sub>GFP</sub> | ||
| Line 24: | Line 23: | ||
|nM | |nM | ||
| | | | ||
| - | |need for | + | |need for 20 measures |
|- | |- | ||
|(''fluorescence'') | |(''fluorescence'') | ||
| Line 30: | Line 29: | ||
|au | |au | ||
| | | | ||
| - | |need for | + | |need for 20 measures |
|- | |- | ||
|''conversion'' | |''conversion'' | ||
| Line 49: | Line 48: | ||
|- | |- | ||
|β<sub>tet</sub> | |β<sub>tet</sub> | ||
| - | | | + | |basal activity of ''pTet'' '''with RBS E0032''' <br> β<sub>16</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| Line 55: | Line 54: | ||
|- | |- | ||
|(K<sub>tet</sub>/{coef<sub>tet</sub>}) | |(K<sub>tet</sub>/{coef<sub>tet</sub>}) | ||
| - | |activation constant of pTet <br> K<sub>13</sub> | + | |activation constant of ''TetR><pTet'' <br> K<sub>13</sub> |
|nM | |nM | ||
| | | | ||
| Line 61: | Line 60: | ||
|- | |- | ||
|n<sub>tet</sub> | |n<sub>tet</sub> | ||
| - | |complexation order of pTet<br> n<sub>13</sub> | + | |complexation order of ''TetR><pTet''<br> n<sub>13</sub> |
|no dimension | |no dimension | ||
| | | | ||
| Line 67: | Line 66: | ||
|- | |- | ||
|K<sub>aTc</sub> | |K<sub>aTc</sub> | ||
| - | |complexation constant aTc | + | |complexation constant ''aTc''><''TetR'' <br> K<sub>12</sub> |
|nM | |nM | ||
| | | | ||
| Line 73: | Line 72: | ||
|- | |- | ||
|n<sub>aTc</sub> | |n<sub>aTc</sub> | ||
| - | |complexation order aTc | + | |complexation order ''aTc><TetR'' <br> n<sub>12</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 12:02, 29 October 2008
| param | signification | unit | value | comments |
| ƒ1 | activity of pTet with RBS E0032 | nM.min-1 | need for 20 values with well choosen [aTc]i | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | Only dilution : Time Cell Division : 35 min. |
| [GFP] | GFP concentration at steady-state | nM | need for 20 measures | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 measures | |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| βtet | basal activity of pTet with RBS E0032 β16 | nM.min-1 | ||
| (Ktet/{coeftet}) | activation constant of TetR><pTet K13 | nM | The optimisation program will give us (γ Ktet / {coeftet} ƒ0) The literature [?] gives Ktet = | |
| ntet | complexation order of TetR><pTet n13 | no dimension | The literature [?] gives ntet = | |
| KaTc | complexation constant aTc><TetR K12 | nM | The literature [?] gives KaTc = | |
| naTc | complexation order aTc><TetR n12 | no dimension | The literature [?] gives naTc = |
