Team:Paris/Modeling/More f1 Table

From 2008.igem.org

(Difference between revisions)
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|comments
|comments
|-
|-
-
|ƒ1
+
|(''fluorescence'')
-
|activity of <br> ''pTet'' '''with RBS E0032'''
+
|value of the observed fluorescence
-
|nM.min<sup>-1</sup>
+
|au
|
|
|need for 20 values with well choosen [aTc]<sub>i</sub> <br>
|need for 20 values with well choosen [aTc]<sub>i</sub> <br>
|-
|-
-
|γ<sub>GFP</sub>
+
|''conversion''
-
|dilution-degradation rate <br> of GFP(mut3b)
+
|conversion ration between <br> fluorescence and concentration <br> &darr; gives &darr;
-
|min<sup>-1</sup>
+
|nM.au<sup>-1</sup>
-
|0.0198
+
|(1/79.429)
-
|Only dilution :<br>Time Cell Division : 35 min.
+
|
|-
|-
|[GFP]
|[GFP]
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|nM
|nM
|
|
-
|need for 20 measures
 
-
|-
 
-
|(''fluorescence'')
 
-
|value of the observed fluorescence
 
-
|au
 
|
|
-
|need for 20 measures
 
|-
|-
-
|''conversion''
+
|γ<sub>GFP</sub>
-
|conversion ration between <br> fluorescence and concentration
+
|dilution-degradation rate <br> of GFP(mut3b) <br> &darr; gives &darr;
-
|nM.au<sup>-1</sup>
+
|min<sup>-1</sup>
-
|(1/79.429)
+
|0.0198
 +
|Only dilution :<br>Time Cell Division : 35 min.
 +
|-
 +
|&#131;1
 +
|activity of <br> ''pTet'' '''with RBS E0032'''
 +
|nM.min<sup>-1</sup>
 +
|
|
|
|}
|}

Revision as of 12:51, 29 October 2008

param signification unit value comments
(fluorescence) value of the observed fluorescence au need for 20 values with well choosen [aTc]i
conversion conversion ration between
fluorescence and concentration
↓ gives ↓ 		nM.au-1 (1/79.429)
[GFP] GFP concentration at steady-state nM
γGFP dilution-degradation rate
of GFP(mut3b)
↓ gives ↓ 		min-1 0.0198 Only dilution :
Time Cell Division : 35 min.
ƒ1 activity of
pTet with RBS E0032 		nM.min-1



param signification
corresponding parameters in the equations 		unit value comments
βtet basal activity of pTet with RBS E0032
β16 		nM.min-1
(Ktet/{coeftet}) activation constant of TetR><pTet
K13 		nM The optimisation program will give us (γ Ktet / {coeftet} ƒ0)
The literature [?] gives Ktet =
ntet complexation order of TetR><pTet
n13 		no dimension The literature [?] gives ntet =
KaTc complexation constant aTc><TetR
K12 		nM The literature [?] gives KaTc =
naTc complexation order aTc><TetR
n12 		no dimension The literature [?] gives naTc =
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