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Team:Paris/Modeling/More f1 Table
From 2008.igem.org
(Difference between revisions)
| Line 48: | Line 48: | ||
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|β<sub>tet</sub> | |β<sub>tet</sub> | ||
| - | |basal activity of ''pTet'' '''with RBS E0032''' <br> β<sub>16</sub> | + | |basal activity of <br> ''pTet'' '''with RBS E0032''' <br> β<sub>16</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>tet</sub>/{coef<sub> | + | |(K<sub>''tet''</sub>/{coef<sub>''tetR''</sub>}) |
|activation constant of ''TetR><pTet'' <br> K<sub>13</sub> | |activation constant of ''TetR><pTet'' <br> K<sub>13</sub> | ||
|nM | |nM | ||
Latest revision as of 13:02, 29 October 2008
| param | signification | unit | value | comments |
| (fluorescence) | value of the observed fluorescence | au | need for 20 values with well choosen [aTc]i | |
| conversion | conversion ration between fluorescence and concentration ↓ gives ↓ | nM.au-1 | (1/79.429) | |
| [GFP] | GFP concentration at steady-state | nM | ||
| γGFP | dilution-degradation rate of GFP(mut3b) ↓ gives ↓ | min-1 | 0.0198 | Only dilution : Time Cell Division : 35 min. |
| ƒ1 | activity of pTet with RBS E0032 | nM.min-1 |
| param | signification corresponding parameters in the equations | unit | value | comments |
| βtet | basal activity of pTet with RBS E0032 β16 | nM.min-1 | ||
| (Ktet/{coeftetR}) | activation constant of TetR><pTet K13 | nM | The optimisation program will give us (γ Ktet / {coeftet} ƒ0) The literature [?] gives Ktet = | |
| ntet | complexation order of TetR><pTet n13 | no dimension | The literature [?] gives ntet = | |
| KaTc | complexation constant aTc><TetR K12 | nM | The literature [?] gives KaTc = | |
| naTc | complexation order aTc><TetR n12 | no dimension | The literature [?] gives naTc = |
