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Team:Paris/Modeling/More f3 Table
From 2008.igem.org
(Difference between revisions)
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|comments | |comments | ||
|- | |- | ||
| - | | | + | |(''fluorescence'') |
| - | | | + | |value of the observed fluorescence |
| - | | | + | |au |
| | | | ||
|need for 20 mesures with well choosen values of [aTc]<sub>i</sub> <br> and for 20 mesures with well choosen values of [arab]<sub>i</sub> <br> and 5x5 measures for the relation below? | |need for 20 mesures with well choosen values of [aTc]<sub>i</sub> <br> and for 20 mesures with well choosen values of [arab]<sub>i</sub> <br> and 5x5 measures for the relation below? | ||
|- | |- | ||
| - | | | + | |''conversion'' |
| - | | | + | |conversion ratio between <br> fluorescence and concentration <br> ↓ gives ↓ |
| - | | | + | |nM.au<sup>-1</sup> |
| - | | | + | |(1/79.429) |
| - | | | + | | |
|- | |- | ||
|[GFP] | |[GFP] | ||
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|nM | |nM | ||
| | | | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| | | | ||
| - | |||
|- | |- | ||
| - | | | + | |γ<sub>GFP</sub> |
| - | | | + | |dilution-degradation rate <br> of GFP(mut3b) <br> ↓ gives ↓ |
| - | | | + | |min<sup>-1</sup> |
| - | | | + | |0.0198 |
| + | |Time Cell Division : 35 min. | ||
| + | |- | ||
| + | |ƒ3 | ||
| + | |activity of <br> ''pFlhDC'' '''with RBS E0032''' | ||
| + | |nM.min<sup>-1</sup> | ||
| + | | | ||
| | | | ||
|} | |} | ||
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{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
|param | |param | ||
| - | |signification <br> corresponding parameters in the | + | |signification <br> corresponding parameters in the <span style="color:#0000FF;">equations</span> |
|unit | |unit | ||
|value | |value | ||
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|- | |- | ||
|β<sub>22</sub> | |β<sub>22</sub> | ||
| - | | | + | |total transcription rate of <br> ''FliA''><''pFlhDC'' '''with RBS B0034''' <br> β<sub>22</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>6</sub>/{coef<sub>fliA</sub>}) | + | |(K<sub>6</sub>/{coef<sub>''fliA''</sub>}) |
|activation constant of ''FliA''><''pFlhDC'' <br> K<sub>6</sub> | |activation constant of ''FliA''><''pFlhDC'' <br> K<sub>6</sub> | ||
|nM | |nM | ||
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|- | |- | ||
|β<sub>17</sub> | |β<sub>17</sub> | ||
| - | |basal activity of ''pFlhDC'' '''with RBS B0034''' <br> β<sub>17</sub> | + | |basal activity of <br> ''pFlhDC'' '''with RBS B0034''' <br> β<sub>17</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub>15</sub>/{coef<sub> | + | |(K<sub>15</sub>/{coef<sub>''ompR''</sub>}) |
|activation constant of ''OmpR''><''pFlhDC'' <br> K<sub>15</sub> | |activation constant of ''OmpR''><''pFlhDC'' <br> K<sub>15</sub> | ||
|nM | |nM | ||
Latest revision as of 13:36, 29 October 2008
| param | signification | unit | value | comments |
| (fluorescence) | value of the observed fluorescence | au | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the relation below? | |
| conversion | conversion ratio between fluorescence and concentration ↓ gives ↓ | nM.au-1 | (1/79.429) | |
| [GFP] | GFP concentration at steady-state | nM | ||
| γGFP | dilution-degradation rate of GFP(mut3b) ↓ gives ↓ | min-1 | 0.0198 | Time Cell Division : 35 min. |
| ƒ3 | activity of pFlhDC with RBS E0032 | nM.min-1 |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β22 | total transcription rate of FliA><pFlhDC with RBS B0034 β22 | nM.min-1 | ||
| (K6/{coeffliA}) | activation constant of FliA><pFlhDC K6 | nM | ||
| n6 | complexation order of FliA><pFlhDC n6 | no dimension | ||
| β17 | basal activity of pFlhDC with RBS B0034 β17 | nM.min-1 | ||
| (K15/{coefompR}) | activation constant of OmpR><pFlhDC K15 | nM | ||
| n15 | complexation order of OmpR><pFlhDC n15 | no dimension |
