Team:Paris/Modeling/Workflow Example

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{{Paris/Header|Generic Workflow on an Example}}
{{Paris/Header|Generic Workflow on an Example}}
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Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the steps of our system. The choosen example is '''the ACTIVITY of the PROMOTER pFliL''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''.
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Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is '''the ACTIVITY of the PROMOTER pFliL''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the TRANSCRIPTION FACTORS FlhDC and FliA'''.
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br>
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br>
As it is illustrated below; starting from <span style="color:#0000FF;">molecular reactions</span> '''(1)''', we develop a <span style="color:#0000FF;">model of our system</span> '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate <span style="color:#0000FF;">Promoters Activities as functions of their Transcription Factors</span>. To do so, we propose <span style="color:#0000FF;">constructs</span> '''(4)''', <span style="color:#0000FF;">protocols</span> '''(5)''' and <span style="color:#0000FF;">programs</span> '''(6)'''.
As it is illustrated below; starting from <span style="color:#0000FF;">molecular reactions</span> '''(1)''', we develop a <span style="color:#0000FF;">model of our system</span> '''(2)''', and then we identify the parameters '''(3)''' that needs to be estimated. Essentially, it amounts to estimate <span style="color:#0000FF;">Promoters Activities as functions of their Transcription Factors</span>. To do so, we propose <span style="color:#0000FF;">constructs</span> '''(4)''', <span style="color:#0000FF;">protocols</span> '''(5)''' and <span style="color:#0000FF;">programs</span> '''(6)'''.
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{{Paris/Toggle|Developpments|Team:Paris/Modeling/Rubriques}}  
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{{Paris/Toggle|Precisions on the Steps|Team:Paris/Modeling/Rubriques}}  
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[[Team:Paris/Modeling/Characterization_Approach| <Back - to the "Presentation"| ]][[Team:Paris/Modeling/Characterization_Approach| |Next - to the "Conclusion"> ]]
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[[Team:Paris/Modeling/Characterization_Approach| <Back - to the "Presentation" ]]|[[Team:Paris/Modeling/Characterization_Approach| Next - to the "Conclusion"> ]]
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Revision as of 16:35, 28 October 2008

Generic Workflow on an Example


Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is the ACTIVITY of the PROMOTER pFliL (that leads to the production of the Fluorescent Protein FP1) in function of the TRANSCRIPTION FACTORS FlhDC and FliA. We know that the hexamere FlhD4C2 and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this phenomenon?
As it is illustrated below; starting from molecular reactions (1), we develop a model of our system (2), and then we identify the parameters (3) that needs to be estimated. Essentially, it amounts to estimate Promoters Activities as functions of their Transcription Factors. To do so, we propose constructs (4), protocols (5) and programs (6).

You can click on the images or in the button below to get further informations on the represented step


Modelisation Experiments








Once we got every parameters for every functions involved in our Full System, we have got a "Modular Virtual Lab" of our System that aims to be predictive.



<Back - to the "Presentation" | Next - to the "Conclusion">

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