Team:Paris/Modeling/Workflow Example

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Generic Workflow on an Example

Here, we will explain the generic protocole that leads to the caracterisation of one of the steps of the system. The choosen example is the EXPRESSION of the PROMOTER pFliL in function of the TRANSCRIPTION FACTORS FlhDC and FliA. We know that the hexamere FlhD4C2 and the protein FliA act both on the promoter pFliL as inducers... how do we evaluate this action ? The following table sums up the equations and the manipulations that could allow us to do it.

Starting from molecular reactions (1) we develop a model of our system (2) we identify the parameters (3) that needs to be estimated. Essentially, it amounts to estimate Promoter Activity as a function of TF. To do so, we propose constructs (4) protocols (5) and programs (6).

Modelisation Principle of experimental caracterisations : we mesure Promoter Activity for various TF concentrations. These various concentrations are obtained using a previously characterised inducible promoter.
bail
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