Team:Paris/Modeling/f0

From 2008.igem.org

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[[Image:F0.jpg|thumb]] In the '''exponential phase of growth''', at the steady state, we have
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{{Paris/Menu}}
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[[Image:eqF0.jpg]]
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{{Paris/Header|Method & Algorithm : ƒ0}}
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[[Image:F0.jpg|thumb|Specific Plasmid Characterisation for ƒ0]] In the '''exponential phase of growth''', at the steady state, we have
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[[Image:eqF0.jpg]]
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<center>
{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
|param
|param
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|unit
|unit
|value
|value
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|comments
|-
|-
|[exprJ23101]
|[exprJ23101]
|expression rate of <br> J23101 '''with RBS E0032'''
|expression rate of <br> J23101 '''with RBS E0032'''
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
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|
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|-
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|dilution-degradation rate <br> of GFP(mut3b)
|dilution-degradation rate <br> of GFP(mut3b)
|min<sup>-1</sup>
|min<sup>-1</sup>
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|0.0198
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|-
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|[GFP]
|[GFP]
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|(1/79.429)
|(1/79.429)
|}
|}
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</center>
That will directly give us &#131;0, which corresponds to &beta;<sub>72</sub> in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.
That will directly give us &#131;0, which corresponds to &beta;<sub>72</sub> in the [[Team:Paris/Modeling/Synchronisation|equations]], if the ''pConstitutive'' is J23101.

Revision as of 08:38, 29 October 2008

Method & Algorithm : ƒ0


Specific Plasmid Characterisation for ƒ0
In the exponential phase of growth, at the steady state, we have

EqF0.jpg

param signification unit value comments
[exprJ23101] expression rate of
J23101 with RBS E0032
nM.min-1
γGFP dilution-degradation rate
of GFP(mut3b)
min-1
[GFP] GFP concentration at steady-state nM
(fluorescence) value of the observed fluorescence au mean value of ~20 measures
conversion conversion ratio between
fluorescence and concentration
nM.au-1 (1/79.429)

That will directly give us ƒ0, which corresponds to β72 in the equations, if the pConstitutive is J23101.