"

 

From 2008.igem.org

In the exponential phase of growth, at the steady state, we have

so, with

that gives, after reducing the expression

with the previous expression of [TetR]_eq

so, finally, if we use a calculated value of [TetR] (by using the expression of ƒ0 for instance, or by introducing a value given by tetR after an other "caracterised promoter")

param	signification	unit	value
[exprJ23101]	expression rate of J23101 with RBS E0032	nM.s-1
γGFP	dilution-degradation rate of GFP(mut3b)	s-1	ln(2)/3600
[GFP]	GFP concentration at steady-state	nM
(fluorescence)	value of the observed fluorescence	au
conversion	conversion ration between fluorescence and concentration	nM.au-1	(1/79.429)

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