Team:Paris/Modeling/f1

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F1a.jpg

The experience would give us

F1expr.jpg

Thus, at steady-state and in the exponential phase of growth :

ExprptetF0.jpg


param signification unit value comments
[expr(pTet)] expression rate of
pTet with RBS E0032
nM.min-1 need for 20 values with well choosen [aTc]i

with 5 measures for each

γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198 Time for Cell Division : 35 min. ; No degradation
[GFP] GFP concentration at steady-state nM need for 100 measures
(fluorescence) value of the observed fluorescence au need for 100 measures
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value comments
βtet production rate of pTet with RBS E0032
β1
nM.min-1
(Ktet/{coeftet}) activation constant of pTet
K20
nM The optimisation program will give us (γ Ktet / {coeftet} ƒ0)
The literature [?] gives Ktet =
ntet complexation order of pTet
n20
no dimension The literature [?] gives ntet =
KaTc complexation constant aTc-TetR
K19
nM The literature [?] gives KaTc =
naTc complexation order aTc-TetR
n19
no dimension The literature [?] gives naTc =

Also, this experiment will allow us to know the expression of ƒ1 :

ExprF1.jpg