Team:Paris/Modeling/f2

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(Difference between revisions)
Line 16: Line 16:
|[expr(pBad)]
|[expr(pBad)]
|expression rate of <br> pBad '''with RBS E0032'''
|expression rate of <br> pBad '''with RBS E0032'''
-
|nM.s<sup>-1</sup>
+
|nM.min<sup>-1</sup>
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 measures
|-
|-
|γ<sub>GFP</sub>
|γ<sub>GFP</sub>
|dilution-degradation rate <br> of GFP(mut3b)
|dilution-degradation rate <br> of GFP(mut3b)
-
|s<sup>-1</sup>
+
|min<sup>-1</sup>
-
|ln(2)/3600
+
|0.0198
|-
|-
|[GFP]
|[GFP]
Line 50: Line 50:
|β<sub>bad</sub>
|β<sub>bad</sub>
|production rate of pBad '''with RBS E0032''' <br> not in the system
|production rate of pBad '''with RBS E0032''' <br> not in the system
-
|nM.s<sup>-1</sup>
+
|nM.min<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>bad</sub>/[AraC<sub>tot</sub>]<sup>n<sub>bad</sub></sup>)
+
|(K<sub>bad</sub>/[AraC<sub>tot</sub>])
|activation constant of pBad <br> not in the system
|activation constant of pBad <br> not in the system
-
|nM<sup>n<sub>bad</sub></sup>
+
|nM
|
|
|-
|-
Line 65: Line 65:
|K<sub>ara</sub>
|K<sub>ara</sub>
|complexation constant Arabinose-AraC <br> not in the system
|complexation constant Arabinose-AraC <br> not in the system
-
|nM<sup>n<sub>ara</sub></sup>
+
|nM
|
|
|-
|-

Revision as of 22:18, 4 September 2008

F2.jpg
According to previous considerations on pBad, we have
F2expr.jpg

so, with the complexation between Arabinose and AraC, and after reducing the equation

F2exprRedu.jpg


param signification unit value
[expr(pBad)] expression rate of
pBad with RBS E0032 		nM.min-1 see "findparam"
need for 20 measures
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 measures
(fluorescence) value of the observed fluorescence au need for 20 measures
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
βbad production rate of pBad with RBS E0032
not in the system 		nM.min-1
(Kbad/[AraCtot]) activation constant of pBad
not in the system 		nM
nbad complexation order of pBad
not in the system 		no dimension
Kara complexation constant Arabinose-AraC
not in the system 		nM
nara complexation order Arabinose-AraC
not in the system 		no dimension
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