Team:Paris/Modeling/f3

From 2008.igem.org

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|β<sub>2</sub>
|β<sub>2</sub>
|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
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|nM.s<sup>-1</sup>
+
|nM.min<sup>-1</sup>
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Revision as of 06:12, 5 September 2008

F3omp.jpg
At the steady-state, we have
FliAeq.jpg

and

OmpReq.jpg

so the expression

F3expr.jpg

gives

F3FliA.jpg

and

F3OmpR.jpg

and for calculated values of the TF,

F3FliACalc.jpg

and

F3OmpRCalc.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β12 production rate of FliA-pFlhDC with RBS E0032
β12
nM.min-1
(K11/{coeffliA}) activation constant of FliA-pFlhDC
K11
nM
n11 complexation order of FliA-pFlhDC
n11
no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2
nM.min-1
(K19/{coefompR}) activation constant of OmpR-pFlhDC
K19
nM
n19 complexation order of OmpR-pFlhDC
n19
no dimension



Then, if we have time, we want to verify the expected relation

SumFlhDC1.jpg