Team:Paris/Modeling/f3

From 2008.igem.org

(Difference between revisions)
Line 1: Line 1:
-
[[Image:f3omp.jpg|thumb]] At the steady-state, we have
+
[[Image:f3omp.jpg|thumb]]
-
<center>[[Image:FliAeq.jpg]]</center>
 
-
and
 
-
<center>[[Image:OmpReq.jpg]]</center>
 
-
so the expression
 
-
 
-
<center>[[Image:f3expr.jpg]]</center>
 
-
 
-
gives
 
-
 
-
<center>[[Image:f3FliA.jpg]]</center>
 
-
and
 
-
<center>[[Image:f3OmpR.jpg]]</center>
 
-
 
-
and for ''calculated values of the TF'',
 
-
 
-
<center>[[Image:f3FliACalc.jpg]]</center>
 
-
and
 
-
<center>[[Image:f3OmpRCalc.jpg]]</center>
 
<br><br>
<br><br>
Line 99: Line 81:
Then, if we have time, we want to verify the expected relation
Then, if we have time, we want to verify the expected relation
-
<center>[[Image:SumFlhDC1.jpg]]</center>
+
[[Image:SumFlhDC1.jpg|center]]

Revision as of 23:44, 29 September 2008

F3omp.jpg




param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032
nM.min-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β12 production rate of FliA-pFlhDC with RBS E0032
β12
nM.min-1
(K11/{coeffliA}) activation constant of FliA-pFlhDC
K11
nM
n11 complexation order of FliA-pFlhDC
n11
no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2
nM.min-1
(K19/{coefompR}) activation constant of OmpR-pFlhDC
K19
nM
n19 complexation order of OmpR-pFlhDC
n19
no dimension



Then, if we have time, we want to verify the expected relation

SumFlhDC1.jpg