Team:Paris/Modeling/f7

From 2008.igem.org

(Difference between revisions)
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|-
|-
|[expr(pFlgA)]
|[expr(pFlgA)]
-
|expression rate of <br> pFlgA '''with RBS E0032'''
+
|expression rate of <br> pFlgB '''with RBS E0032'''
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|value
|value
|-
|-
-
|β<sub>55</sub>
+
|β<sub>57</sub>
-
|production rate of FlhDC-pFlgA '''with RBS E0032''' <br> β<sub>55</sub>
+
|production rate of FlhDC-pFlgB '''with RBS E0032''' <br> β<sub>57</sub>
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>49</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>49</sub></sup>)
+
|(K<sub>51</sub>/{coef<sub>flhDC</sub>}<sup>n<sub>51</sub></sup>)
-
|activation constant of FlhDC-pFlgA <br> K<sub>49</sub>
+
|activation constant of FlhDC-pFlgB <br> K<sub>51</sub>
-
|nM<sup>n<sub>49</sub></sup>
+
|nM<sup>n<sub>51</sub></sup>
|
|
|-
|-
-
|n<sub>49</sub>
+
|n<sub>51</sub>
-
|complexation order of FliA-pFlgA <br> n<sub>49</sub>
+
|complexation order of FliA-pFlgB <br> n<sub>51</sub>
|no dimension
|no dimension
|
|
|-
|-
|-
|-
-
|β<sub>56</sub>
+
|β<sub>58</sub>
-
|production rate of FliA-pFlgA '''with RBS E0032''' <br> β<sub>56</sub>
+
|production rate of FliA-pFlgB '''with RBS E0032''' <br> β<sub>52</sub>
|nM.s<sup>-1</sup>
|nM.s<sup>-1</sup>
|
|
|-
|-
-
|(K<sub>50</sub>/{coef<sub>fliA</sub>}<sup>n<sub>50</sub></sup>)
+
|(K<sub>52</sub>/{coef<sub>fliA</sub>}<sup>n<sub>52</sub></sup>)
-
|activation constant of FliA-pFlgA <br> K<sub>50</sub>
+
|activation constant of FliA-pFlgA <br> K<sub>52</sub>
-
|nM<sup>n<sub>50</sub></sup>
+
|nM<sup>n<sub>52</sub></sup>
|
|
|-
|-
-
|n<sub>50</sub>
+
|n<sub>52</sub>
-
|complexation order of FliA-pFlgA <br> n<sub>50</sub>
+
|complexation order of FliA-pFlgB <br> n<sub>52</sub>
|no dimension
|no dimension
|
|

Revision as of 17:04, 21 August 2008

F7DCA.png
At the steady-state, we have
FlhDCeq.jpg

and

FliAeq.jpg

so the expression

F7expr.jpg

gives

F7FlhDC.jpg

and

F7FliA.jpg

and for calculated values of the TF,

F7FlhDCCalc.jpg

and

F7FliACalc.jpg



param signification unit value
[expr(pFlgA)] expression rate of
pFlgB with RBS E0032
nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b)
s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration
nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations
unit value
β57 production rate of FlhDC-pFlgB with RBS E0032
β57
nM.s-1
(K51/{coefflhDC}n51) activation constant of FlhDC-pFlgB
K51
nMn51
n51 complexation order of FliA-pFlgB
n51
no dimension
β58 production rate of FliA-pFlgB with RBS E0032
β52
nM.s-1
(K52/{coeffliA}n52) activation constant of FliA-pFlgA
K52
nMn52
n52 complexation order of FliA-pFlgB
n52
no dimension



Then, if we have time, we want to verify the expected relation

SumpFlgB.jpg