Team:Paris/Network analysis and design/System improvements

From 2008.igem.org

(Difference between revisions)
(System Improvements)
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We need a 'delay' so we can:
We need a 'delay' so we can:
 +
1 add more genes to the circuit
1 add more genes to the circuit
 +
2 use quorum sensing (qs)
2 use quorum sensing (qs)
we chose quorum sensing that can allowed both 'delay' and population synchonization.
we chose quorum sensing that can allowed both 'delay' and population synchonization.
-
two ways to add it: S4 and S2.
+
two ways to add it:  
-
We first analyse S4 due to modularity.
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*S4: Inspired from Garcia-Ojalvo: oscillating repressilator + oscillating qs module = synchronized oscillations
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Inspired from Garcia-Ojalvo: oscillating repressilator + oscillating qs module = synchronized oscillations
+
*S2: it amounts to replace single interaction by more complex interactions: should create delay
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we tried s4:
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 +
We first analyse S4 due to modularity.
damped oscillating core system + oscillating qs module = damped oscillations.
damped oscillating core system + oscillating qs module = damped oscillations.

Revision as of 21:58, 25 October 2008

System Improvements
Name Description Evaluation Links
Core system Figure core system Zero Liks core system
Modular system Figure modular system ** Links Garcia-Ojalvo
Resulting system Figure resulting system *** Links resulting
Main conclusion: we keep S2


Core system does not oscillate. How can we enhance it?

We need a 'delay' so we can:

1 add more genes to the circuit

2 use quorum sensing (qs)

we chose quorum sensing that can allowed both 'delay' and population synchonization. two ways to add it:

  • S4: Inspired from Garcia-Ojalvo: oscillating repressilator + oscillating qs module = synchronized oscillations
  • S2: it amounts to replace single interaction by more complex interactions: should create delay


We first analyse S4 due to modularity.

damped oscillating core system + oscillating qs module = damped oscillations.

We continue with S2:

S0 module in S4 gived us insides in parameters analysis for S2.

given analysis results we keep S2.