Team:Paris/Notebook/Freezer

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Paris

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
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Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Biobricks Description Products in use Date
MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 (clones 1 + 2) 2007
MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 (clones 1 + 2) MP2.2 2007
MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator (clones 1 + 2) MP3.3, MP3.4 2007
MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD (clones 1 + 2) 2007
MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.png Strong RBS (clones 1 + 2) 2007
MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term) (clones 1 + 2) MP6'.1, MP6'.2 2007
MP8 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp tri-part; strong rbs (clones 1 + 2) 2007


Plasmids: MiniPrep "in preparation"
Number Name Biobricks Description Products in use Date
[http://partsregistry.org/Part:BBa_B0034 B0034] Part icon rbs.png Strongest RBS 2008
[http://partsregistry.org/Part:BBa_C0040 C0040] Icon coding.png tetR repressor 2008
[http://partsregistry.org/Part:BBa_C0079 C0079] Icon coding.png lasR activator with LVA Tag 2008
[http://partsregistry.org/Part:BBa_C0179 C0179] Icon coding.png lasR activator 2007
[http://partsregistry.org/Part:BBa_E0030 E0030] Part icon reporter.png YFP 2008
[http://partsregistry.org/Part:BBa_E0040 E0040] Part icon reporter.png GFP 2008
[http://partsregistry.org/Part:BBa_E1010 E1010] Part icon reporter.png mRFP 2008
[http://partsregistry.org/Part:BBa_J23101 J23101] Part icon regulatory.png Strongest Constitutive promoter 2008
[http://partsregistry.org/Part:BBa_J23109 J23109] Part icon regulatory.png Weak Constitutive promoter 2008
[http://partsregistry.org/Part:BBa_R0040 R0040] Part icon regulatory.png tetR repressible promoter 2008
[http://partsregistry.org/Part:BBa_R0079 R0079] Part icon regulatory.png promoter pLas (lasr & PAI regulated) 2008
[http://partsregistry.org/Part:BBa_S03154 S03154] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI 2008
[http://partsregistry.org/Part:BBa_S03879 S03879] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR 2008

Digestion Products


PURPLE = Vector
GREEN = Insert

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left []ng/µL Date
D1 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 34 July 17
D2 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 887 27 July 17